Adhesion, growth, and matrix production by fibroblasts on laminin substrates

J. R. Couchman, M. Hook, D. A. Rees, R. Timpl

Research output: Contribution to journalArticle

120 Scopus citations

Abstract

Human embryonic skin fibroblasts have been shown to attach and spread on laminin substrates in the absence of protein synthesis and presence of fibronectin-depleted serum and anti-fibronectin antibodies. Rates of attachment and the type of spreading are virtually identical on fibronectin and laminin-coated substrates with the development of microfilament bundles and focal adhesions. Antibodies to laminin, but not fibronectin, will prevent or reverse fibroblast adhesion to laminin, whereas antibodies to fibronectin but not laminin will give similar results on fibronectin-coated substrates. These and other results indicate that fibroblasts possess distinct receptors for laminin and fibronectin which on contact with suitable substrates promote adhesion through interaction with common intermediates. This type of adhesion is compatible with subsequent growth and extracellular matrix production.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalJournal of Cell Biology
Volume96
Issue number1
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Cell Biology

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