Adenovirus-mediated thymidine kinase gene transduction in human epithelial ovarian cancer cell lines followed by exposure to ganciclovir

X. W. Tong, A. Block, S. H. Chen, S. L C Woo, D. G. Kieback

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

In an effort to develop gene therapy for ovarian cancer efficacy and toxicity of adenovirus-mediated transfer of the HSV-TK gene followed by administration of ganciclovir were studied in two human epithelial ovarian cancer cell lines Ov-ca-2774 and Ov-ca-1225. 100% transduction was achieved in both cell lines at MOIs of 7 and 15 as demonstrated by X-Gal staining. No toxicity of virus alone was observed at MOIs up to 30. GCV was not toxic up to 200 μg/ml. Cell killing efficacy was shown to be dependent on MOI as well as GCV dose. The 'bystander effect' of ADV/RSV-TK was quantified by mixing experiments and found to be dependent on the proportion of ADV/RSV-TK positive cells as well as the GCV dosage. Similar results were observed in both cell lines. ADV/RSV-TK mediated gene therapy may be a promising approach in ovarian cancer.

Original languageEnglish (US)
Pages (from-to)1611-1617
Number of pages7
JournalAnticancer Research
Volume16
Issue number4 A
StatePublished - Jul 1 1996

Keywords

  • Adenovirus-TK
  • Gene therapy
  • Ovarian cancer
  • Suicide genes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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