Adeno-associated virus type 2 vector-mediated glial cell line-derived neurotrophic factor gene transfer induces neuroprotection and neuroregeneration in a ubiquitin-proteasome system impairment animal model of Parkinson's disease

Yunlan Du, Xiaojie Zhang, Qingqing Tao, Sheng Chen, Weidong Le

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Background: The impairment of the ubiquitin-proteasome system (UPS) is a cellular mechanism underlying the neurodegenerative process in Parkinson's disease (PD). A mouse model induced by the selective proteasome inhibitor lactacystin targeting on substantia nigra has been demonstrated to be valuable in investigating etiopathogenesis and neuroprotection for PD. Objective: In the present study, we used adeno-associated virus type 2 vector (AAV2) encoding glial cell line-derived neurotrophic factor (GDNF) injected into the striatum of this animal model to test the effectiveness and possible mechanisms of GDNF gene therapy. Results: Our results showed that AAV2-mediated GDNF gene therapy significantly attenuated lactacystin-induced loss of nigral dopamine (DA) neurons and striatal DA levels. Furthermore, we found that GDNF protein is mostly expressed in astrocytes in the subventricular zone (SVZ) and dentate gyrus (DG). AAV2-mediated GDNF therapy can induce neurogenesis in the SVZ and DG, and increase the number of nigral newborn DA neurons. Conclusion: These data indicate that AAV2-mediated GDNF gene therapy can protect the nigral DA neurons from the UPS impairment-induced degeneration, which may partly result from the nigral DA neuron regeneration in the brain, and such experimental results may have implications for the treatment of PD.

Original languageEnglish (US)
Pages (from-to)113-128
Number of pages16
JournalNeurodegenerative Diseases
Volume11
Issue number3
DOIs
StatePublished - Jan 2013

Keywords

  • Gene therapy
  • Glial cell line-derived neurotrophic factor
  • Neuroregeneration
  • Parkinson's disease
  • Proteasome inhibitor

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

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