Addicting diverse bacteria to a noncanonical amino acid

Drew S. Tack; Jared W. Ellefson; Ross Thyer; Bo Wang; Jimmy Gollihar; Matthew T. Forster; Andrew D. Ellington

doi:10.1038/nchembio.2002

Addicting diverse bacteria to a noncanonical amino acid

Drew S. Tack, Jared W. Ellefson, Ross Thyer, Bo Wang, Jimmy Gollihar, Matthew T. Forster, Andrew D. Ellington

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Engineered orthogonal translation systems have greatly enabled the expansion of the genetic code using noncanonical amino acids (NCAAs). However, the impact of NCAAs on organismal evolution remains unclear, in part because it is difficult to force the adoption of new genetic codes in organisms. By reengineering TEM-1 β-lactamase to be dependent on a NCAA, we maintained bacterial NCAA dependence for hundreds of generations without escape.

Original language	English (US)
Pages (from-to)	138-140
Number of pages	3
Journal	Nature Chemical Biology
Volume	12
Issue number	3
DOIs	https://doi.org/10.1038/nchembio.2002
State	Published - Feb 1 2016

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1038/nchembio.2002

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abstract = "Engineered orthogonal translation systems have greatly enabled the expansion of the genetic code using noncanonical amino acids (NCAAs). However, the impact of NCAAs on organismal evolution remains unclear, in part because it is difficult to force the adoption of new genetic codes in organisms. By reengineering TEM-1 β-lactamase to be dependent on a NCAA, we maintained bacterial NCAA dependence for hundreds of generations without escape.",

author = "Tack, \{Drew S.\} and Ellefson, \{Jared W.\} and Ross Thyer and Bo Wang and Jimmy Gollihar and Forster, \{Matthew T.\} and Ellington, \{Andrew D.\}",

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AU - Ellington, Andrew D.

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N2 - Engineered orthogonal translation systems have greatly enabled the expansion of the genetic code using noncanonical amino acids (NCAAs). However, the impact of NCAAs on organismal evolution remains unclear, in part because it is difficult to force the adoption of new genetic codes in organisms. By reengineering TEM-1 β-lactamase to be dependent on a NCAA, we maintained bacterial NCAA dependence for hundreds of generations without escape.

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Addicting diverse bacteria to a noncanonical amino acid

Abstract

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