Addicting diverse bacteria to a noncanonical amino acid

Drew S. Tack, Jared W. Ellefson, Ross Thyer, Bo Wang, Jimmy Gollihar, Matthew T. Forster, Andrew D. Ellington

Research output: Contribution to journalArticlepeer-review

48 Scopus citations


Engineered orthogonal translation systems have greatly enabled the expansion of the genetic code using noncanonical amino acids (NCAAs). However, the impact of NCAAs on organismal evolution remains unclear, in part because it is difficult to force the adoption of new genetic codes in organisms. By reengineering TEM-1 β-lactamase to be dependent on a NCAA, we maintained bacterial NCAA dependence for hundreds of generations without escape.

Original languageEnglish (US)
Pages (from-to)138-140
Number of pages3
JournalNature Chemical Biology
Issue number3
StatePublished - Feb 1 2016

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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