Acute Exposure to Pyridostigmine Bromide Disrupts Cholinergic Myenteric Neuroimmune Function in Mice

Claudia A. Collier, Steven Foncerrada, Abigail J. Clevenger, Ashok Shetty, Shreya A. Raghavan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Gulf War Illness (GWI) results from chemical exposure during the Gulf War, with notable impacts on gastrointestinal motility. Due to the limited demographic impacted by this ailment, an in-depth investigation of the GWI has yielded little regarding the underlying pathophysiological mechanisms. Here, the hypothesis that exposure to pyridostigmine bromide (PB) results in severe enteric neuro-inflammation, that cascades to disruptions in colonic motility, is tested. The analyses are performed on male C57BL/6 mice that are treated with physiologically similar doses of PB given to GW veterans. When colonic motility is assessed, GWI colons have significantly reduced forces in response to acetylcholine or electrical field stimulation. GWI is also accompanied by high levels of pro-inflammatory cytokines and chemokines, associated with increased numbers of CD40+ pro-inflammatory macrophages within the myenteric plexus. Enteric neurons responsible for mediating colonic motility reside within the myenteric plexus, and PB exposure reduced their numbers. Significant smooth muscle hypertrophy is also observed due to increased inflammation. Together, the results show that PB exposure caused functional and anatomical dysfunction, promoting impaired motility within the colon. Achieving a greater understanding of the mechanisms of GWI will allow more refinement in therapeutic options that improve veterans’ quality of life.

Original languageEnglish (US)
Article number2200254
Pages (from-to)e2200254
JournalAdvanced Biology
Issue number5
StatePublished - May 2023


  • enteric nervous system
  • gastroi ntestinal tract
  • gulf war illness
  • myenteric neurons
  • neuroinf lammation
  • pyrido stigmine bromide
  • tissue engineering
  • Inflammation/chemically induced
  • Mice, Inbred C57BL
  • Male
  • Animals
  • Persian Gulf Syndrome/chemically induced
  • Quality of Life
  • Cholinesterase Inhibitors/toxicity
  • Mice
  • Pyridostigmine Bromide/pharmacology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biomedical Engineering
  • Biomaterials


Dive into the research topics of 'Acute Exposure to Pyridostigmine Bromide Disrupts Cholinergic Myenteric Neuroimmune Function in Mice'. Together they form a unique fingerprint.

Cite this