Activation of the Cbl insulin signaling pathway in cardiac muscle; Dysregulation in obesity and diabetes

Anisha Gupte, Silvia Mora

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

In adipocytes, the Cbl/CAP dependent signaling pathway has been involved in regulating insulin-stimulated glucose uptake. We investigated activation of Cbl and its downstream effector TC10 in cardiac and skeletal muscle of Balb/C mice. Insulin administration resulted in Cbl phosphorylation in cardiac, skeletal muscle, and adipose tissue. Subsequent TC10 activation was detected only in heart and adipose tissue. c-Cbl and CAP gene expression was significantly reduced in the heart tissue of streptozotocin-induced diabetic animals, whereas no change was observed for other components of the pathway. No changes in Cbl expression were detected in hindlimb muscle. In leptin-/- obese mice Cbl expression in heart and adipose tissue was maintained, although insulin-mediated Cbl phosphorylation and subsequent TC10 activation were significantly reduced. In conclusion, our data demonstrate that Cbl/CAP/TC10 insulin signaling pathway is active in cardiac muscle and impaired during obesity and insulin deficiency.

Original languageEnglish (US)
Pages (from-to)751-757
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume342
Issue number3
DOIs
StatePublished - Apr 14 2006

Keywords

  • Cbl
  • Diabetes
  • Heart
  • Insulin
  • Obesity
  • TC10

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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