TY - JOUR
T1 - Activation, aggregation and adhesion of platelets exposed to high-intensity focused ultrasound
AU - Poliachik, Sandra L.
AU - Chandler, Wayne L.
AU - Mourad, Pierre D.
AU - Ollos, Ryan J.
AU - Crum, Lawrence A.
N1 - Funding Information:
This research was supported by DARPA via the Office of Naval Research, contract number N00014-96-1-0630. The authors wish to thank Dorothy Lowell and Dong Qin at the University of Washington for their helpful assistance.
PY - 2001
Y1 - 2001
N2 - Using platelet-rich plasma, we investigated the effect of 1.1-MHz continuous wave high-intensity focused ultrasound (HIFU) on platelet activation, aggregation and adhesion to a collagen-coated surface. Platelets were exposed for durations of 10-500 s at spatial average intensities of up to 4860 W/cm2. To avoid heating effects, the average temperature in the HIFU tank was maintained at 33.8 ± 4.0°C during platelet experiments. Flow cytometry, laser aggregometry, environmental scanning electron microscopy and passive cavitation detection were used to observe and to quantify platelet activation, aggregation, adhesion to a collagen-coated surface and associated cavitation. It was determined that HIFU can activate platelets, stimulate them to aggregate and promote their adherence to a collagen-coated surface. In principle, HIFU can stimulate primary, or platelet-related, hemostasis. Cavitation was monitored by a passive cavitation detector during aggregation trials and was quantified to provide a relative measure of the amount of cavitation that occurred in each aggregation trial. Regression analysis shows a weak correlation (r2 = 0.11) between aggregation and ultrasound intensity, but a substantial correlation (r2 = 0.76) between aggregation and cavitation occurrence.
AB - Using platelet-rich plasma, we investigated the effect of 1.1-MHz continuous wave high-intensity focused ultrasound (HIFU) on platelet activation, aggregation and adhesion to a collagen-coated surface. Platelets were exposed for durations of 10-500 s at spatial average intensities of up to 4860 W/cm2. To avoid heating effects, the average temperature in the HIFU tank was maintained at 33.8 ± 4.0°C during platelet experiments. Flow cytometry, laser aggregometry, environmental scanning electron microscopy and passive cavitation detection were used to observe and to quantify platelet activation, aggregation, adhesion to a collagen-coated surface and associated cavitation. It was determined that HIFU can activate platelets, stimulate them to aggregate and promote their adherence to a collagen-coated surface. In principle, HIFU can stimulate primary, or platelet-related, hemostasis. Cavitation was monitored by a passive cavitation detector during aggregation trials and was quantified to provide a relative measure of the amount of cavitation that occurred in each aggregation trial. Regression analysis shows a weak correlation (r2 = 0.11) between aggregation and ultrasound intensity, but a substantial correlation (r2 = 0.76) between aggregation and cavitation occurrence.
KW - Aggregation
KW - Bioeffects
KW - Cavitation
KW - HIFU
KW - Platelets
KW - Ultrasound
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U2 - 10.1016/S0301-5629(01)00444-6
DO - 10.1016/S0301-5629(01)00444-6
M3 - Article
C2 - 11750756
AN - SCOPUS:0035668618
SN - 0301-5629
VL - 27
SP - 1567
EP - 1576
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 11
ER -