Activation, aggregation and adhesion of platelets exposed to high-intensity focused ultrasound

Sandra L. Poliachik, Wayne L. Chandler, Pierre D. Mourad, Ryan J. Ollos, Lawrence A. Crum

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


Using platelet-rich plasma, we investigated the effect of 1.1-MHz continuous wave high-intensity focused ultrasound (HIFU) on platelet activation, aggregation and adhesion to a collagen-coated surface. Platelets were exposed for durations of 10-500 s at spatial average intensities of up to 4860 W/cm2. To avoid heating effects, the average temperature in the HIFU tank was maintained at 33.8 ± 4.0°C during platelet experiments. Flow cytometry, laser aggregometry, environmental scanning electron microscopy and passive cavitation detection were used to observe and to quantify platelet activation, aggregation, adhesion to a collagen-coated surface and associated cavitation. It was determined that HIFU can activate platelets, stimulate them to aggregate and promote their adherence to a collagen-coated surface. In principle, HIFU can stimulate primary, or platelet-related, hemostasis. Cavitation was monitored by a passive cavitation detector during aggregation trials and was quantified to provide a relative measure of the amount of cavitation that occurred in each aggregation trial. Regression analysis shows a weak correlation (r2 = 0.11) between aggregation and ultrasound intensity, but a substantial correlation (r2 = 0.76) between aggregation and cavitation occurrence.

Original languageEnglish (US)
Pages (from-to)1567-1576
Number of pages10
JournalUltrasound in Medicine and Biology
Issue number11
StatePublished - 2001


  • Aggregation
  • Bioeffects
  • Cavitation
  • HIFU
  • Platelets
  • Ultrasound

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


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