TY - JOUR
T1 - Activation, aggregation and adhesion of platelets exposed to high intensity focused ultrasound
AU - Poliachik, Sandra L.
AU - Chandler, Wayne L.
AU - Mourad, Pierre D.
AU - Ollos, Ryan J.
AU - Crum, Lawrence A.
PY - 2000
Y1 - 2000
N2 - High intensity focused ultrasound (HIFU) is being considered as a non-invasive method to halt internal bleeding, thus we investigated the capability of HIFU to produce "acoustic primary hemostasis," including platelet activation, aggregation and adhesion to a collagen-coated surface. Various HIFU doses were applied to platelet rich plasma (PRP) with and without ultrasound contrast agents (UCA). Flow cytometry, laser aggregometry, environmental scanning electron microscopy (ESEM) and passive cavitation detection were used to quantify platelet activation, aggregation, adhesion and associated cavitation. HIFU can activate platelets and cause them to adhere to a collagen-coated surface. Cavitation was monitored during aggregation trials and was quantified to provide a relative measure of the amount of cavitation that occurred in each aggregation trial. Regression analysis shows weak correlation between aggregation and intensity, and a strong correlation between aggregation and cavitation occurrence.
AB - High intensity focused ultrasound (HIFU) is being considered as a non-invasive method to halt internal bleeding, thus we investigated the capability of HIFU to produce "acoustic primary hemostasis," including platelet activation, aggregation and adhesion to a collagen-coated surface. Various HIFU doses were applied to platelet rich plasma (PRP) with and without ultrasound contrast agents (UCA). Flow cytometry, laser aggregometry, environmental scanning electron microscopy (ESEM) and passive cavitation detection were used to quantify platelet activation, aggregation, adhesion and associated cavitation. HIFU can activate platelets and cause them to adhere to a collagen-coated surface. Cavitation was monitored during aggregation trials and was quantified to provide a relative measure of the amount of cavitation that occurred in each aggregation trial. Regression analysis shows weak correlation between aggregation and intensity, and a strong correlation between aggregation and cavitation occurrence.
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M3 - Conference article
AN - SCOPUS:0034579309
VL - 2
SP - 1433
EP - 1436
JO - Proceedings of the IEEE Ultrasonics Symposium
JF - Proceedings of the IEEE Ultrasonics Symposium
SN - 1051-0117
T2 - 2000 IEEE Ultrasonics Symposium
Y2 - 22 October 2000 through 25 October 2000
ER -