Acquisition of the heat-shock response and thermotolerance during early development of Xenopus laevis

John J. Heikkila, Malgorzata Kloc, Jeff Bury, Gilbert A. Schultz, Leon W. Browder

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

The ability to synthesize a 68,000- to 70,000-Da protein (hsp) in heat-shocked early Xenopus laevis embryos is dependent on the stage of development. Whereas late blastula and later stage embryos synthesize hsp68-70 after heat shock, cleavage stages are incompetent with respect to hsp synthesis. In vitro translation experiments and RNA blot analyses demonstrate that enhanced synthesis of hsp68-70 is associated with an accumulation of hsp68-70 mRNA. Examination of the effect of heat shock on preexisting actin mRNA reveals that heat shock promotes a reduction in the levels of actin mRNA in cleavage embryos but has no discernible effect on actin mRNA levels in neurula embryos. Finally, the acquisition of the heat-shock response (i.e., synthesis of hsp68-70 and accumulation of hsp70 mRNA) during early Xenopus development is correlated with the acquisition of thermotolerance.

Original languageEnglish (US)
Pages (from-to)483-489
Number of pages7
JournalDevelopmental Biology
Volume107
Issue number2
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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