TY - JOUR
T1 - Acquisition of the heat-shock response and thermotolerance during early development of Xenopus laevis
AU - Heikkila, John J.
AU - Kloc, Malgorzata
AU - Bury, Jeff
AU - Schultz, Gilbert A.
AU - Browder, Leon W.
N1 - Funding Information:
This research has been supported by a Medical Research Council (MRC) of Canada postdoctoral fellowship to J.J.H. along with a research allowancef rom the Alberta Heritage Foundation for Medical Research (AHFMR), a grant from MRC to G.A.S., an AHFMR research fellowship to M.K., and a grant from the Natural Sciences and Engineering Research Council to L.W.B. The authors are grateful to Mrs. Jillian Wilkes for her excellent technical assistance.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1985/2
Y1 - 1985/2
N2 - The ability to synthesize a 68,000- to 70,000-Da protein (hsp) in heat-shocked early Xenopus laevis embryos is dependent on the stage of development. Whereas late blastula and later stage embryos synthesize hsp68-70 after heat shock, cleavage stages are incompetent with respect to hsp synthesis. In vitro translation experiments and RNA blot analyses demonstrate that enhanced synthesis of hsp68-70 is associated with an accumulation of hsp68-70 mRNA. Examination of the effect of heat shock on preexisting actin mRNA reveals that heat shock promotes a reduction in the levels of actin mRNA in cleavage embryos but has no discernible effect on actin mRNA levels in neurula embryos. Finally, the acquisition of the heat-shock response (i.e., synthesis of hsp68-70 and accumulation of hsp70 mRNA) during early Xenopus development is correlated with the acquisition of thermotolerance.
AB - The ability to synthesize a 68,000- to 70,000-Da protein (hsp) in heat-shocked early Xenopus laevis embryos is dependent on the stage of development. Whereas late blastula and later stage embryos synthesize hsp68-70 after heat shock, cleavage stages are incompetent with respect to hsp synthesis. In vitro translation experiments and RNA blot analyses demonstrate that enhanced synthesis of hsp68-70 is associated with an accumulation of hsp68-70 mRNA. Examination of the effect of heat shock on preexisting actin mRNA reveals that heat shock promotes a reduction in the levels of actin mRNA in cleavage embryos but has no discernible effect on actin mRNA levels in neurula embryos. Finally, the acquisition of the heat-shock response (i.e., synthesis of hsp68-70 and accumulation of hsp70 mRNA) during early Xenopus development is correlated with the acquisition of thermotolerance.
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U2 - 10.1016/0012-1606(85)90329-X
DO - 10.1016/0012-1606(85)90329-X
M3 - Article
C2 - 3972166
AN - SCOPUS:0021917408
SN - 0012-1606
VL - 107
SP - 483
EP - 489
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -