Acetonitrile-based solvents: Their application in thin-layer chromatography of cyclic nucleotides, nucleosides, purine, and pyrimidines

Joseph F. Tomashefski, Roberto J. Barrios, Oscar Sudilovsky

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Acetonitrile-based solvent mixtures have been applied to the separation of nucleotides, nucleosides, purines, pyrimidines, and cyclic nucleotides by thin-layer chromatography. The Rf's of over 35 compounds are presented. Development times with some of the systems were as low as 16 min. The use of acetonitrile-containing solvents in adenyl cyclase and cyclic phosphodiesterase assays and in the nucleotides and nucleic acid fields is discussed.

Original languageEnglish (US)
Pages (from-to)589-595
Number of pages7
JournalAnalytical Biochemistry
Volume60
Issue number2
DOIs
StatePublished - Aug 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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