TY - JOUR
T1 - Accessory nuclei revisited
T2 - The translocation of snRNPs from the germinal vesicle to the periphery of the future embryo
AU - Biliński, Szczepan M.
AU - Kloc, Małgorzata
N1 - Funding Information:
Acknowledgements We thank Prof. J.G. Gall (Carnegie Institution, Baltimore, Md., USA) for Y12 monoclonal antibody, U5 and U6 snRNA plasmids, Prof. E.K.L. Chan (Scripps Research Institute, La Jolla, Calif., USA) for an antibody against coilin (R288) and Prof. M. Yoshida (University of Tokyo, Japan) for leptomycin B. We also want to thank Prof. L.D. Etkin (University of Texas, Houston, Tex., USA) for support and encouragement and Prof. W. Kilar-ski (Jagiellonian University, Kraków, Poland) for providing EM facilities. We would like to acknowledge the excellent technical assistance of Mrs. Beata Szymańska and Władysława Jankowska. This study was supported by research grants DS/IZ/ZS/00 and 6P04C 110 21 from the State Committee for Scientific Research (KBN; to S.M.B.) and by grant GM 50221 from NIH to Laurence D. Etkin. The described experiments comply with the current laws of Poland.
PY - 2002/3
Y1 - 2002/3
N2 - Oocytes of certain insects contain peculiar organelles termed accessory nuclei (AN). These organelles originate by budding off from the envelope of the oocyte nucleus and contain 1-2 dense inclusions immersed in a translucent ground substance. We have demonstrated that in the wasp Vespula germanica each inclusion consists of two elements: a spherical body, and a hemispherical structure composed of numerous 20-30 nm particles. Immunoelectron microscopy and whole-mount in situ hybridization have shown that the inclusions contain AgNOR-staining proteins, p80-coilin, Sm proteins, and small nuclear RNAs (snRNAs). These results indicate that the inclusions and hemispherical structures are homologous to Cajal bodies and B-snurposomes of Xenopus germinal vesicles, respectively. During previtellogenesis, AN (together with their Cajal bodies) migrate to the cortical ooplasm of the oocyte where they reside at least until the onset of embryogenesis. We suggest that AN are vehicles for the transport and localization of snRNPs to the periphery of the oocyte, i.e., to the region where the blastoderm of the embryo develops and where there is a requirement for a high concentration of RNA-processing factors.
AB - Oocytes of certain insects contain peculiar organelles termed accessory nuclei (AN). These organelles originate by budding off from the envelope of the oocyte nucleus and contain 1-2 dense inclusions immersed in a translucent ground substance. We have demonstrated that in the wasp Vespula germanica each inclusion consists of two elements: a spherical body, and a hemispherical structure composed of numerous 20-30 nm particles. Immunoelectron microscopy and whole-mount in situ hybridization have shown that the inclusions contain AgNOR-staining proteins, p80-coilin, Sm proteins, and small nuclear RNAs (snRNAs). These results indicate that the inclusions and hemispherical structures are homologous to Cajal bodies and B-snurposomes of Xenopus germinal vesicles, respectively. During previtellogenesis, AN (together with their Cajal bodies) migrate to the cortical ooplasm of the oocyte where they reside at least until the onset of embryogenesis. We suggest that AN are vehicles for the transport and localization of snRNPs to the periphery of the oocyte, i.e., to the region where the blastoderm of the embryo develops and where there is a requirement for a high concentration of RNA-processing factors.
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U2 - 10.1007/s00412-002-0186-4
DO - 10.1007/s00412-002-0186-4
M3 - Article
C2 - 12068924
AN - SCOPUS:0036490479
SN - 0009-5915
VL - 111
SP - 62
EP - 68
JO - Chromosoma
JF - Chromosoma
IS - 1
ER -