TY - JOUR
T1 - Absolute mRNA levels and transcriptional regulation of the mouse testis-specific thioredoxins
AU - Jiménez, Alberto
AU - Prieto-Álamo, María J.
AU - Fuentes-Almagro, Carlos A.
AU - Jurado, Juan
AU - Gustafsson, Jan Åke
AU - Pueyo, Carmen
AU - Miranda-Vizuete, Antonio
N1 - Funding Information:
This work was supported by the Swedish Medical Research Council (Projects 03P-14096, 03X-14041, and 13X-10370), the Åke Wibergs Stiftelse, the Karolinska Institutet, and the Spanish Ministerio de Ciencia y Tecnología (Grant BMC2002-00179). A. Jiménez was supported by a postdoctoral fellowship (EX2003-0390) from the Spanish Ministerio de Educación, Cultura y Deporte. M.-J. Prieto-Álamo and J. Jurado were recipients of postdoctoral contracts (Programa Ramón y Cajal) from the Spanish Ministerio de Ciencia y Tecnología.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2005/4/29
Y1 - 2005/4/29
N2 - Thioredoxins function as general protein disulphide reductases. Mammalian male germ cells are equipped with a set of three testis-specific thioredoxins (named Sptrx-1, -2, and -3, respectively) that are expressed either in different structures within the sperm cell or at different stages of sperm development. Previous studies based on qualitative northern-blot and in situ hybridization analyses restricted the presence of Sptrx mRNAs to adult testis, but nothing is known about their transcriptional regulation or relative expression levels in this tissue. In this report, we investigate the transcriptional profiles of the mouse Sptrx genes in terms of the germ cell-specific regulation by promoter analysis in GC-2spd(ts) cells. Besides, we perform a comprehensive quantification of the Sptrx mRNA molecules by real-time PCR in whole-animal experiments. By these means, we show that transcription is differentially regulated for each Sptrx gene and identify the 5′-flanking regions anticipated to contain the cis-regulatory elements responsible, at least in part, for the transcriptional silencing and/or activation of the Sptrx genes. In addition, we show remarkable age-associated variations between the Sptrx mRNA expression patterns.
AB - Thioredoxins function as general protein disulphide reductases. Mammalian male germ cells are equipped with a set of three testis-specific thioredoxins (named Sptrx-1, -2, and -3, respectively) that are expressed either in different structures within the sperm cell or at different stages of sperm development. Previous studies based on qualitative northern-blot and in situ hybridization analyses restricted the presence of Sptrx mRNAs to adult testis, but nothing is known about their transcriptional regulation or relative expression levels in this tissue. In this report, we investigate the transcriptional profiles of the mouse Sptrx genes in terms of the germ cell-specific regulation by promoter analysis in GC-2spd(ts) cells. Besides, we perform a comprehensive quantification of the Sptrx mRNA molecules by real-time PCR in whole-animal experiments. By these means, we show that transcription is differentially regulated for each Sptrx gene and identify the 5′-flanking regions anticipated to contain the cis-regulatory elements responsible, at least in part, for the transcriptional silencing and/or activation of the Sptrx genes. In addition, we show remarkable age-associated variations between the Sptrx mRNA expression patterns.
KW - Gene expression
KW - Promoter
KW - Real-time PCR
KW - Sperm
KW - Testis
KW - Thioredoxin
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U2 - 10.1016/j.bbrc.2005.02.128
DO - 10.1016/j.bbrc.2005.02.128
M3 - Article
C2 - 15781233
AN - SCOPUS:15544374111
SN - 0006-291X
VL - 330
SP - 65
EP - 74
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -