Absence of prognostic significance of bcl-2 immunopositivity in non- small cell lung cancer: Analysis of 427 cases

Rose Anton, Richard W. Brown, Mamoun Younes, Margaret M. Gondo, Mark A. Stephenson, Philip T. Cagle

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52 Scopus citations


The bcl-2 gene product inhibits apoptosis and is thought to participate in oncogenesis. Association of bcl-2 immunopositivity with improved prognosis of non-small cell lung cancers (NSCLG) is controversial. Although two studies have reported better survival in bcl-2immunopositive NSCLCs, a third series has contradicted this finding. The authors studied a relatively larger case series involving 427 patients for whom detailed information on long-term follow-up was available to determine the prognostic significance of bcl-2 expression. The study included 252 adenocarcinomas (AC), 111 squamous cell carcinomas (SCG), and 64 large cell carcinomas (LC). After antigen retrieval, sections were immunostained using a monoclonal anti-bcl2 antibody (1:60, Clone 124, Dako) and the avidin-bintin complex technique. Staining was scored as positive or negative mad also on a semiquantitative scale as 0, low (<10%), moderate (10% to 75%), or extensive (>75%). Bcl-2 immunoreactivity was correlated with survival using the actuarial survival method, Kaplan- Meier method, and log-rank test and was not associated with statistically significant differences in survival for NSCLCs (P = .5537). Differences in survival remained insignificant even after NSCLCs were stratified for cell type, stage, or grade, singly or in combination. Therefore, using this method, bcl-2 immunopositivity does not appear to act as an independent prognostic indicator in NSCLCs.

Original languageEnglish (US)
Pages (from-to)1079-1082
Number of pages4
JournalHuman Pathology
Issue number9
StatePublished - Jan 1 1997


  • Bcl-2
  • Immunohistochemistry
  • Lung
  • Non-small cell carcinoma
  • Prognostic factors

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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