A vertebral skeletal stem cell lineage driving metastasis

Jun Sun, Lingling Hu, Seoyeon Bok, Alisha R. Yallowitz, Michelle Cung, Jason McCormick, Ling J. Zheng, Shawon Debnath, Yuzhe Niu, Adrian Y. Tan, Sarfaraz Lalani, Kyle W. Morse, Daniel Shinn, Anthony Pajak, Mohammed Hammad, Vincentius Jeremy Suhardi, Zan Li, Na Li, Lijun Wang, Weiguo ZouVivek Mittal, Mathias P.G. Bostrom, Ren Xu, Sravisht Iyer, Matthew B. Greenblatt

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Vertebral bone is subject to a distinct set of disease processes from long bones, including a much higher rate of solid tumour metastases 1–4. The basis for this distinct biology of vertebral bone has so far remained unknown. Here we identify a vertebral skeletal stem cell (vSSC) that co-expresses ZIC1 and PAX1 together with additional cell surface markers. vSSCs display formal evidence of stemness, including self-renewal, label retention and sitting at the apex of their differentiation hierarchy. vSSCs are physiologic mediators of vertebral bone formation, as genetic blockade of the ability of vSSCs to generate osteoblasts results in defects in the vertebral neural arch and body. Human counterparts of vSSCs can be identified in vertebral endplate specimens and display a conserved differentiation hierarchy and stemness features. Multiple lines of evidence indicate that vSSCs contribute to the high rates of vertebral metastatic tropism observed in breast cancer, owing in part to increased secretion of the novel metastatic trophic factor MFGE8. Together, our results indicate that vSSCs are distinct from other skeletal stem cells and mediate the unique physiology and pathology of vertebrae, including contributing to the high rate of vertebral metastasis.

Original languageEnglish (US)
Pages (from-to)602-609
Number of pages8
Issue number7979
StatePublished - Sep 21 2023

ASJC Scopus subject areas

  • General


Dive into the research topics of 'A vertebral skeletal stem cell lineage driving metastasis'. Together they form a unique fingerprint.

Cite this