A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants

Eliot H. Frank; Moonsoo Jin; Andreas M. Loening; Marc E. Levenston; Alan J. Grodzinsky

doi:10.1016/S0021-9290(00)00100-7

A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants

Eliot H. Frank, Moonsoo Jin, Andreas M. Loening, Marc E. Levenston, Alan J. Grodzinsky

Research output: Contribution to journal › Article › peer-review

147 Scopus citations

Abstract

We have developed an incubator housed, biaxial-tissue-loading device capable of applying axial deformations as small as 1 μm and sinusoidal rotations as small as 0.01°. Axial resolution is 50 nm for applying sinewaves as low as 10 μm (or 1% based on a 1 mm thickness) or as large as 100 μm. Rotational resolution is 0.0005°. The machine is small enough (30 cm high x 25 cm x 20 cm) to be placed in a standard incubator for long-term tissue culture loading studies. In metabolic studies described here, application of sinusoidal macroscopic shear deformation to articular cartilage explants resulted in a significant increase in the synthesis of proteoglycan and proteins (uptake of ³⁵S-sulfate and ³H-proline) over controls held at the same static offset compression. (C) 2000 Elsevier Science Ltd.

Original language	English (US)
Pages (from-to)	1523-1527
Number of pages	5
Journal	Journal of Biomechanics
Volume	33
Issue number	11
DOIs	https://doi.org/10.1016/S0021-9290(00)00100-7
State	Published - Nov 1 2000

Keywords

Cartilage biomechanics
Chondrocyte metabolism
Mechanotransduction
Shear
Tissue loading

ASJC Scopus subject areas

Biophysics
Orthopedics and Sports Medicine
Biomedical Engineering
Rehabilitation

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10.1016/S0021-9290(00)00100-7

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title = "A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants",

abstract = "We have developed an incubator housed, biaxial-tissue-loading device capable of applying axial deformations as small as 1 μm and sinusoidal rotations as small as 0.01°. Axial resolution is 50 nm for applying sinewaves as low as 10 μm (or 1% based on a 1 mm thickness) or as large as 100 μm. Rotational resolution is 0.0005°. The machine is small enough (30 cm high x 25 cm x 20 cm) to be placed in a standard incubator for long-term tissue culture loading studies. In metabolic studies described here, application of sinusoidal macroscopic shear deformation to articular cartilage explants resulted in a significant increase in the synthesis of proteoglycan and proteins (uptake of 35S-sulfate and 3H-proline) over controls held at the same static offset compression. (C) 2000 Elsevier Science Ltd.",

keywords = "Cartilage biomechanics, Chondrocyte metabolism, Mechanotransduction, Shear, Tissue loading",

author = "Frank, {Eliot H.} and Moonsoo Jin and Loening, {Andreas M.} and Levenston, {Marc E.} and Grodzinsky, {Alan J.}",

note = "Funding Information: This work was supported by NIH Grants AR33236 and AR45779 and an Arthritis Foundation Postdoctoral Fellowship to M.E. Levenston. ",

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doi = "10.1016/S0021-9290(00)00100-7",

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AU - Frank, Eliot H.

AU - Jin, Moonsoo

AU - Loening, Andreas M.

AU - Levenston, Marc E.

AU - Grodzinsky, Alan J.

N1 - Funding Information: This work was supported by NIH Grants AR33236 and AR45779 and an Arthritis Foundation Postdoctoral Fellowship to M.E. Levenston.

PY - 2000/11/1

Y1 - 2000/11/1

N2 - We have developed an incubator housed, biaxial-tissue-loading device capable of applying axial deformations as small as 1 μm and sinusoidal rotations as small as 0.01°. Axial resolution is 50 nm for applying sinewaves as low as 10 μm (or 1% based on a 1 mm thickness) or as large as 100 μm. Rotational resolution is 0.0005°. The machine is small enough (30 cm high x 25 cm x 20 cm) to be placed in a standard incubator for long-term tissue culture loading studies. In metabolic studies described here, application of sinusoidal macroscopic shear deformation to articular cartilage explants resulted in a significant increase in the synthesis of proteoglycan and proteins (uptake of 35S-sulfate and 3H-proline) over controls held at the same static offset compression. (C) 2000 Elsevier Science Ltd.

AB - We have developed an incubator housed, biaxial-tissue-loading device capable of applying axial deformations as small as 1 μm and sinusoidal rotations as small as 0.01°. Axial resolution is 50 nm for applying sinewaves as low as 10 μm (or 1% based on a 1 mm thickness) or as large as 100 μm. Rotational resolution is 0.0005°. The machine is small enough (30 cm high x 25 cm x 20 cm) to be placed in a standard incubator for long-term tissue culture loading studies. In metabolic studies described here, application of sinusoidal macroscopic shear deformation to articular cartilage explants resulted in a significant increase in the synthesis of proteoglycan and proteins (uptake of 35S-sulfate and 3H-proline) over controls held at the same static offset compression. (C) 2000 Elsevier Science Ltd.

KW - Cartilage biomechanics

KW - Chondrocyte metabolism

KW - Mechanotransduction

KW - Shear

KW - Tissue loading

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A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants

Abstract

Keywords

ASJC Scopus subject areas

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