TY - JOUR
T1 - A transcript map of the chromosome 19q-arm glioma tumor suppressor region
AU - Smith, Justin S.
AU - Tachibana, Issei
AU - Pohl, Ute
AU - Lee, Hyun K.
AU - Thanarajasingam, Uma
AU - Portier, Bryce P.
AU - Ueki, Keisuke
AU - Ramaswamy, Shivapriya
AU - Billings, Stephanie J.
AU - Mohrenweiser, Harvey W.
AU - Louis, David N.
AU - Jenkins, Robert B.
N1 - Funding Information:
We thank the members of the Mayo Sequencing Core for their diligent efforts and helpful suggestions with regard to BAC sequencing. This work was supported by NIH Grants CA 50905 (R.B.J.) and CA 69285 (D.N.L.) and by the National Brain Tumor Foundation (J.S.S., D.N.L., and R.B.J.). Work performed by the Lawrence Livermore National Laboratory is under the auspices of the U.S. Department of Energy, Contract W-7405-Eng-48. Justin Smith is supported by an American Brain Tumor Association Summer Fellowship and by the Sidney Luckman Endowed Physician Scientist Scholarship. Issei Tachibana is supported by the Lindsey Bock Foundation Fellowship, and Ute Pohl is a fellow of the Deutsche For-schungsgemeinschaft.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2000/2/15
Y1 - 2000/2/15
N2 - Allelic loss of the chromosome 19q arm is a frequent event in human diffuse gliomas, suggesting that it contains a tumor suppressor gene. Recent deletion mapping studies have broadly implicated a 1.6-Mb interval between D19S241E and D19S596, with a limited subset of tumors, suggesting that the region may be as narrow as 150 kb. Focusing on this smaller interval, we have used cDNA selection, exon amplification, and genomic sequencing to identify three novel transcripts (EHD2, GLTSCR1, and GLTSCR2) and to map two known genes (SEPW1 and CRX). A partial transcript map of 19 transcripts and two EST markers has been constructed for the 1.6-Mb interval D19S241E-D19S596. Ten of these transcripts, including the 5 mapped to the 150-kb deletion interval, have been examined for alterations in a panel of gliomas with allelic loss of 19q. Tumor-specific alterations have not been identified in the transcripts examined thus far. Collectively, these data should facilitate subsequent efforts to identify and characterize the remaining transcripts in the 1.6-Mb interval. (C) 2000 Academic Press.
AB - Allelic loss of the chromosome 19q arm is a frequent event in human diffuse gliomas, suggesting that it contains a tumor suppressor gene. Recent deletion mapping studies have broadly implicated a 1.6-Mb interval between D19S241E and D19S596, with a limited subset of tumors, suggesting that the region may be as narrow as 150 kb. Focusing on this smaller interval, we have used cDNA selection, exon amplification, and genomic sequencing to identify three novel transcripts (EHD2, GLTSCR1, and GLTSCR2) and to map two known genes (SEPW1 and CRX). A partial transcript map of 19 transcripts and two EST markers has been constructed for the 1.6-Mb interval D19S241E-D19S596. Ten of these transcripts, including the 5 mapped to the 150-kb deletion interval, have been examined for alterations in a panel of gliomas with allelic loss of 19q. Tumor-specific alterations have not been identified in the transcripts examined thus far. Collectively, these data should facilitate subsequent efforts to identify and characterize the remaining transcripts in the 1.6-Mb interval. (C) 2000 Academic Press.
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U2 - 10.1006/geno.1999.6101
DO - 10.1006/geno.1999.6101
M3 - Article
C2 - 10708517
AN - SCOPUS:0034652751
VL - 64
SP - 44
EP - 50
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 1
ER -