A tolerogenic semimature dendritic cells induce effector T-cell hyporesponsiveness by activation of antigen-specific CD4+CD25+ T regulatory cells that promotes skin allograft survival in mice

Bi mang Fu, Xiao shun He, Si Yu, An bin Hu, Jie Zhang, Yi Ma, Nga lei Tam, Jie fu Huang

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Semimature dendritic cells (smDCs) can induce autoimmune tolerance by activation of host antigen-specific CD4+CD25+ regulatory T (Treg) cells. We hypothesized that donor smDCs injected into recipients would induce effector T-cell hyporesponsiveness by activating CD4+CD25+Treg cells, and promote skin allograft survival. Myeloid smDCs were derived from C57BL/6J mice (donors) in vitro. BALB/c mice (recipients) were injected with smDCs to generate antigen-specific CD4+CD25+Treg cells in vivo. Allograft survival was prolonged when BALB/c recipients received either C57BL/6J smDCs prior to grafting or C57BL/6J smDC-derived CD4+CD25+Treg cells post-grafting, and skin flaps from these grafts showed the highest IL-10 production regardless of rapamycin treatments. Our findings confirm that smDCs constitute an independent subgroup of DCs that play a key role for inducing CD4+CD25+Treg cells to express high IL-10 levels, which induce hyporesponsiveness of effector T cells. Pre-treating recipients with donor smDCs may have potential for transplant tolerance induction.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalCellular Immunology
Volume261
Issue number1
DOIs
StatePublished - 2010

Keywords

  • CD4CD25 T regulatory cells
  • Effector T cells
  • Hyporesponsiveness
  • Semimature dendritic cells

ASJC Scopus subject areas

  • Immunology

Fingerprint

Dive into the research topics of 'A tolerogenic semimature dendritic cells induce effector T-cell hyporesponsiveness by activation of antigen-specific CD4+CD25+ T regulatory cells that promotes skin allograft survival in mice'. Together they form a unique fingerprint.

Cite this