A three-dimensional model of primary bovine endometrium using an electrospun scaffold

S. B. MacKintosh, L. P. Serino, P. D. Iddon, R. Brown, R. S. Conlan, C. J. Wright, T. G.G. Maffeis, M. J. Raxworthy, I. M. Sheldon

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Endometrial stromal and epithelial cell function is typically studied in vitro using standard twodimensional monocultures, but these cultures fail to reflect the complex three-dimensional (3D) architecture of tissue. A 3Dmodel of bovine endometrium that reflects the architectural arrangement of in vivo tissue would beneficially assist the study of tissue function. An electrospun polyglycolide (PGA) scaffold was selected to grow a 3Dmodel of primary bovine endometrial epithelial and stromal cells, that reflects the architecture of the endometrium for the study of pathophysiology. Electrospun scaffolds were seeded with stromal and epithelial cells, and growth was assessed using histological techniques. Prostaglandin E2 and prostaglandin F responsiveness of endometrial scaffold constructs was tested using oxytocin plus arachidonic acid (OT + AA) or lipopolysaccharide (LPS). Stromal and epithelial cells growing on the electrospun scaffold had an architectural arrangement that mimicked whole tissue, deposited fibronectin, had appropriate expression of vimentin and cytokeratin and were responsive toOT+ AA and LPS, as measured by prostaglandin accumulation. In conclusion, a functional 3D model of stromal and epithelial cells was developed using a PGA electrospun scaffold which may be used to study endometrial pathophysiology.

Original languageEnglish (US)
Article number025010
JournalBiofabrication
Volume7
Issue number2
DOIs
StatePublished - May 28 2015

Keywords

  • Co-culture
  • Endometrium
  • In vitro
  • Polyglycolide
  • SEM
  • Scaffold
  • Uterus

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

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