A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)

Huiru Lu, Huatang Zhang, Jun Chen, Jinchao Zhang, Ruochuan Liu, Hongyan Sun, Yuliang Zhao, Zhifang Chai, Yi Hu

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

In this study, Cu(II)-mediated differential alteration of cysteine (Cys) reactivity is reported by using a Cys-specific fluorescent probe. The probe could react with Cys to give out strong fluorescence. When Cys was preincubated with Cu(II), the fluorescence of the probe was decreased due to the inhibition of Cys's reactivity by Cu(II). Remarkably, experimental results reveal that the probe could detect Cu(II) at subnanomolar concentrations. In contrast, Cu(II) could only partially inhibit the reaction between Cys and Ellman's reagent (DTNB). Furthermore, selectivity experiments show that Cu(II) is a much more potent inhibitor for Cys compared to other metal ions. Cell imaging experiments also confirm the inhibitory effects of Cu(II) on Cys's reactivity in living cells. We envision that the probe could add a useful tool for sensitive and selective detection of Cu(II) for biomedical research.

Original languageEnglish (US)
Pages (from-to)477-482
Number of pages6
JournalTalanta
Volume146
DOIs
StatePublished - Jan 1 2016

Keywords

  • Cell imaging
  • Copper
  • Cysteine
  • Ellman's reagent
  • Fluorescent probe

ASJC Scopus subject areas

  • Chemistry(all)

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