A synthetic peptide mediated active targeting of cisplatin liposomes to Tie2 expressing cells

Junhua Mai, Shuxian Song, Mengjie Rui, Dan Liu, Qian Ding, Jinliang Peng, Yuhong Xu

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Tie2 receptor is a receptor tyrosine kinase that plays important roles in vascular angiogenesis, and also highly expressed by a number of cancer cells. In this study, we reported an active targeting liposome system directed by a novel peptide ligand PH1 that can improve drug efficacies specifically to Tie2 expressing cells. The PH1 peptide (TMGFTAPRFPHY) was selected by phage display library screening combined with surface plasmon resonance binding assays. It was covalently conjugated to the distal end of DSPE-PEG2000-Maleimide lipid and loaded onto liposome membranes as the targeting ligand. These PH1-PEG-liposomes containing the anticancer drug cisplatin were showed to bind tightly to Tie2 positive cells, mediate active endocytosis of the drug containing liposomes, and result in much higher cell specific cytoxicities than mPEG coated liposomes. They can be used not only to target vascular endothelial cells for anti-angiogenesis effects, but also to improve drug delivery and release in Tie2 expressing cancer cells. Such liposome formulation may be developed into a very useful agent for metronomic chemotherapy.

Original languageEnglish (US)
Pages (from-to)174-181
Number of pages8
JournalJournal of Controlled Release
Volume139
Issue number3
DOIs
StatePublished - Nov 3 2009

Keywords

  • Angiogenesis
  • Liposome
  • Peptide ligand
  • Tie2
  • Tumor targeting

ASJC Scopus subject areas

  • Pharmaceutical Science

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