TY - JOUR
T1 - A superparamagnetic Fe3O4-loaded polymeric nanocarrier for targeted delivery of evodiamine with enhanced antitumor efficacy
AU - Lv, Yanyun
AU - Ding, Guobin
AU - Zhai, Jinghui
AU - Guo, Yi
AU - Nie, Guangjun
AU - Xu, Li
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/10/1
Y1 - 2013/10/1
N2 - The aim of this study was to design and synthesize a polymeric nanocarrier system loaded with both superparamagnetic iron oxide nanoparticles (SPIONs) and the anticancer drug evodiamine through a solvent evaporation technique. The hydrodynamic diameter of the prepared SPION-evodiamine-loaded nanocarrier was approximately 261. nm, and the drug-loading content and encapsulation efficiency were 8.61. ±. 0.73% and 40.36. ±. 3.42%, respectively. The nanocarrier exhibited good superparamagnetism and an iron content of approximately 9.34%. In vitro drug release experiments showed a sustained release profile over 70. h. Staining with Prussian blue confirmed that the nanocarrier could be effectively internalized into HeLa cells. MTT assays indicated that the SPION-evodiamine-loaded nanocarrier showed cytotoxicity comparable to that of free evodiamine. If an external magnetic field was applied, the SPION-loaded nanocarrier accumulated at the targeted sites and demonstrated a magnetic force-mediated targeting property with the aid of a magnetic field. Furthermore, the SPION-evodiamine-loaded nanocarrier exhibited a much higher in vivo antitumor efficacy than free evodiamine. Together, these results indicate that the SPION-evodiamine-loaded nanocarrier could effectively inhibit tumor growth both in vitro and in vivo with reduced toxicity, and therefore is a promising candidate to achieve enhanced therapeutic efficacy for clinical development.
AB - The aim of this study was to design and synthesize a polymeric nanocarrier system loaded with both superparamagnetic iron oxide nanoparticles (SPIONs) and the anticancer drug evodiamine through a solvent evaporation technique. The hydrodynamic diameter of the prepared SPION-evodiamine-loaded nanocarrier was approximately 261. nm, and the drug-loading content and encapsulation efficiency were 8.61. ±. 0.73% and 40.36. ±. 3.42%, respectively. The nanocarrier exhibited good superparamagnetism and an iron content of approximately 9.34%. In vitro drug release experiments showed a sustained release profile over 70. h. Staining with Prussian blue confirmed that the nanocarrier could be effectively internalized into HeLa cells. MTT assays indicated that the SPION-evodiamine-loaded nanocarrier showed cytotoxicity comparable to that of free evodiamine. If an external magnetic field was applied, the SPION-loaded nanocarrier accumulated at the targeted sites and demonstrated a magnetic force-mediated targeting property with the aid of a magnetic field. Furthermore, the SPION-evodiamine-loaded nanocarrier exhibited a much higher in vivo antitumor efficacy than free evodiamine. Together, these results indicate that the SPION-evodiamine-loaded nanocarrier could effectively inhibit tumor growth both in vitro and in vivo with reduced toxicity, and therefore is a promising candidate to achieve enhanced therapeutic efficacy for clinical development.
KW - Amphiphilic copolymer
KW - Cellular uptake
KW - Magnetic nanocarrier
KW - Magnetite nanoparticles
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U2 - 10.1016/j.colsurfb.2013.04.038
DO - 10.1016/j.colsurfb.2013.04.038
M3 - Article
C2 - 23759382
AN - SCOPUS:84879481141
VL - 110
SP - 411
EP - 418
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -