A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

Silvia Masciarelli; Roberto Quaranta; Ilaria Iosue; Gianni Colotti; Fabrizio Padula; Greta Varchi; Francesco Fazi; Alberto Del Rio

doi:10.1021/cb500286b

A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

Silvia Masciarelli, Roberto Quaranta, Ilaria Iosue, Gianni Colotti, Fabrizio Padula, Greta Varchi, Francesco Fazi, Alberto Del Rio

Houston Methodist

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid.

Original language	English (US)
Pages (from-to)	1674-1679
Number of pages	6
Journal	ACS Chemical Biology
Volume	9
Issue number	8
DOIs	https://doi.org/10.1021/cb500286b
State	Published - Aug 15 2014

ASJC Scopus subject areas

Biochemistry
Molecular Medicine

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A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4. / Masciarelli, Silvia; Quaranta, Roberto; Iosue, Ilaria et al.
In: ACS Chemical Biology, Vol. 9, No. 8, 15.08.2014, p. 1674-1679.

Research output: Contribution to journal › Article › peer-review

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AU - Iosue, Ilaria

AU - Colotti, Gianni

AU - Padula, Fabrizio

AU - Varchi, Greta

AU - Fazi, Francesco

AU - Del Rio, Alberto

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A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

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