A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

Silvia Masciarelli, Roberto Quaranta, Ilaria Iosue, Gianni Colotti, Fabrizio Padula, Greta Varchi, Francesco Fazi, Alberto Del Rio

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid.

Original languageEnglish (US)
Pages (from-to)1674-1679
Number of pages6
JournalACS Chemical Biology
Volume9
Issue number8
DOIs
StatePublished - Aug 15 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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