TY - JOUR
T1 - A single cyclin A gene and multiple cyclin B1-related sequences are dispersed in the mouse genome
AU - Lock, Leslie F.
AU - Pines, Jonathan
AU - Hunter, Tony
AU - Gilbert, Debra J.
AU - Gopalan, Ganesan
AU - Jenkins, Nancy A.
AU - Copeland, Neal G.
AU - Donovan, Peter J.
N1 - Funding Information:
We thank B. A. Cho, D. A. Swing, K. Heston, and M. K. Ernst for excellent technical assistance: Drs. A. M. Buchberg, J. D. Ceci, M. J. Justice, K. J. Moore, U. Francke, and L. D. Siracusa for valuable discussion; L. D. Brubaker for word processing assistance; and Dr. M. J. Justice for reviewing the manuscript. We also thank Drs. E. M. Either, T. H. Finlay, L. F. Parada, B. R. Stanton, B. A. Taylor, and S. M. Tilghman for sharing their resources. This research was supported by the National Cancer Institute, DHHS, under Contract NOl-CO-74101 with ABL.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/6
Y1 - 1992/6
N2 - Cyclin activation of protein serine/threonine kinases plays a pivotal role in regulating the cell cycle. Multiple cyclins that fall into at least five classes, A, B, C, D, and E, have been identified. In some organisms, more than one member of a single cyclin class has been observed. To gain insight into the function of cyclin multiplicity, we determined the number of cyclin A- and B1-related sequences present in the mouse genome, the relationship between these cyclin-related sequences and previously described mutations in the mouse, and cyclin A and B1 mRNA expression in mouse embryos. By genetic mapping using human cyclin A and B1 probes, we identified 1 cyclin A gene located on chromosome 3 and 10 cyclin B1-related sequences located on chromosomes 4, 5, 7, 8, 13, 14, 15, and 17. Cyclin B1-related sequences map in the vicinity of the metaphase-arrest mutation oligosyndactyly (Os) and embryonic lethal mutations associated with the albino (c) locus and the t-complex. In Northern analysis, two cyclin A-related transcripts of 2.1 and 3.4 kb and three cyclin B1-related transcripts of 1.7, 2.1, and 2.7 kb were detected in embryonic stem cells and postimplantation embryos from Day 9.5 to 15.5 of development. Identification of multiple cyclin B1-related sequences in the mouse genome and multiple cyclin B1 mRNAs raises the possibility that seemingly redundant cyclin B genes might have developmental- and/or cell-type-specific functions.
AB - Cyclin activation of protein serine/threonine kinases plays a pivotal role in regulating the cell cycle. Multiple cyclins that fall into at least five classes, A, B, C, D, and E, have been identified. In some organisms, more than one member of a single cyclin class has been observed. To gain insight into the function of cyclin multiplicity, we determined the number of cyclin A- and B1-related sequences present in the mouse genome, the relationship between these cyclin-related sequences and previously described mutations in the mouse, and cyclin A and B1 mRNA expression in mouse embryos. By genetic mapping using human cyclin A and B1 probes, we identified 1 cyclin A gene located on chromosome 3 and 10 cyclin B1-related sequences located on chromosomes 4, 5, 7, 8, 13, 14, 15, and 17. Cyclin B1-related sequences map in the vicinity of the metaphase-arrest mutation oligosyndactyly (Os) and embryonic lethal mutations associated with the albino (c) locus and the t-complex. In Northern analysis, two cyclin A-related transcripts of 2.1 and 3.4 kb and three cyclin B1-related transcripts of 1.7, 2.1, and 2.7 kb were detected in embryonic stem cells and postimplantation embryos from Day 9.5 to 15.5 of development. Identification of multiple cyclin B1-related sequences in the mouse genome and multiple cyclin B1 mRNAs raises the possibility that seemingly redundant cyclin B genes might have developmental- and/or cell-type-specific functions.
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U2 - 10.1016/0888-7543(92)90262-Q
DO - 10.1016/0888-7543(92)90262-Q
M3 - Article
C2 - 1535334
AN - SCOPUS:0026753977
SN - 0888-7543
VL - 13
SP - 415
EP - 424
JO - Genomics
JF - Genomics
IS - 2
ER -