A shape analysis method to detect dendritic spine in 3D optical microscopy image

Xiaoyin Xu; Jie Cheng; Rochelle M. Witt; Bernardo L. Sabatini; Stephen T. Wong

A shape analysis method to detect dendritic spine in 3D optical microscopy image

Xiaoyin Xu, Jie Cheng, Rochelle M. Witt, Bernardo L. Sabatini, Stephen T. Wong

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Dendritic spines play an important role in synaptic transmission. Spines are small protrusions formed on the shafts of dendrites and they are manifest of many neurological conditions. Three dimensional images of dendrites and spines are obtained by confocal and two- b photon laser scanning microscopy. We present an image processing method based on shape analysis to detect spines and separate spines from the dendrites for quantitative study. The method applies two grassfire procedures to first find the tips of spines and then reversely locate the boundary between spines and dendrites. The boundary is determined as the propagation front that undergoes the maximum change in plane area of the grassfire procedure. We use real data to demonstrate the performance of the method.

Original language	English (US)
Title of host publication	2006 3rd IEEE International Symposium on Biomedical Imaging
Subtitle of host publication	From Nano to Macro - Proceedings
Pages	554-557
Number of pages	4
Volume	2006
State	Published - Nov 17 2006
Event	2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Arlington, VA, United States Duration: Apr 6 2006 → Apr 9 2006

Other

Other	2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro
Country/Territory	United States
City	Arlington, VA
Period	4/6/06 → 4/9/06

ASJC Scopus subject areas

General Engineering

Cite this

Xu, X, Cheng, J, Witt, RM, Sabatini, BL & Wong, ST 2006, A shape analysis method to detect dendritic spine in 3D optical microscopy image. in 2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings. vol. 2006, 1624976, pp. 554-557, 2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Arlington, VA, United States, 4/6/06.

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title = "A shape analysis method to detect dendritic spine in 3D optical microscopy image",

abstract = "Dendritic spines play an important role in synaptic transmission. Spines are small protrusions formed on the shafts of dendrites and they are manifest of many neurological conditions. Three dimensional images of dendrites and spines are obtained by confocal and two- b photon laser scanning microscopy. We present an image processing method based on shape analysis to detect spines and separate spines from the dendrites for quantitative study. The method applies two grassfire procedures to first find the tips of spines and then reversely locate the boundary between spines and dendrites. The boundary is determined as the propagation front that undergoes the maximum change in plane area of the grassfire procedure. We use real data to demonstrate the performance of the method.",

author = "Xiaoyin Xu and Jie Cheng and Witt, {Rochelle M.} and Sabatini, {Bernardo L.} and Wong, {Stephen T.}",

year = "2006",

month = nov,

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language = "English (US)",

isbn = "0780395778",

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T1 - A shape analysis method to detect dendritic spine in 3D optical microscopy image

AU - Xu, Xiaoyin

AU - Cheng, Jie

AU - Witt, Rochelle M.

AU - Sabatini, Bernardo L.

AU - Wong, Stephen T.

PY - 2006/11/17

Y1 - 2006/11/17

N2 - Dendritic spines play an important role in synaptic transmission. Spines are small protrusions formed on the shafts of dendrites and they are manifest of many neurological conditions. Three dimensional images of dendrites and spines are obtained by confocal and two- b photon laser scanning microscopy. We present an image processing method based on shape analysis to detect spines and separate spines from the dendrites for quantitative study. The method applies two grassfire procedures to first find the tips of spines and then reversely locate the boundary between spines and dendrites. The boundary is determined as the propagation front that undergoes the maximum change in plane area of the grassfire procedure. We use real data to demonstrate the performance of the method.

AB - Dendritic spines play an important role in synaptic transmission. Spines are small protrusions formed on the shafts of dendrites and they are manifest of many neurological conditions. Three dimensional images of dendrites and spines are obtained by confocal and two- b photon laser scanning microscopy. We present an image processing method based on shape analysis to detect spines and separate spines from the dendrites for quantitative study. The method applies two grassfire procedures to first find the tips of spines and then reversely locate the boundary between spines and dendrites. The boundary is determined as the propagation front that undergoes the maximum change in plane area of the grassfire procedure. We use real data to demonstrate the performance of the method.

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M3 - Conference contribution

AN - SCOPUS:33750961236

SN - 0780395778

SN - 9780780395770

VL - 2006

SP - 554

EP - 557

BT - 2006 3rd IEEE International Symposium on Biomedical Imaging

T2 - 2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro

Y2 - 6 April 2006 through 9 April 2006

ER -

A shape analysis method to detect dendritic spine in 3D optical microscopy image

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