TY - JOUR
T1 - A Sensitive Reporter Cell Line for HIV-1 tat Activity, HIV-1 Inhibitors, and T Cell Activation Effects
AU - Aguilar-Cordova, Estuardo
AU - Chinen, Javier
AU - Donehower, Larry
AU - Lewis, Dorothy E.
AU - Belmont, John W.
PY - 1994/3
Y1 - 1994/3
N2 - The production and characterization of a cell line for quantitative HIV-1 Tat function and T cell activation assays is described. 1G5 is a clonal cell line derived from Jurkat T cells stably transfected with a luciferase gene driven by an HIV-1 long terminal repeat (HIV-LTR). The 1G5 clone was selected for low basal luciferase activity, susceptibility to HIV infection, and high responsiveness to Tat and T cell activation signals. A 10 to 1000-fold increase in luciferase activity after transfection or infection with tat-expressing vectors or HIV was observed. Equivalent levels of expression were detected after stimulation with T cell mitogens. The characteristics of 1G5 make it a valuable reagent for studies of HIV infection, HIV regulatory agents, and other T cell or HIV -activating factors, and for screening potential anti-HIV therapeutic agents.
AB - The production and characterization of a cell line for quantitative HIV-1 Tat function and T cell activation assays is described. 1G5 is a clonal cell line derived from Jurkat T cells stably transfected with a luciferase gene driven by an HIV-1 long terminal repeat (HIV-LTR). The 1G5 clone was selected for low basal luciferase activity, susceptibility to HIV infection, and high responsiveness to Tat and T cell activation signals. A 10 to 1000-fold increase in luciferase activity after transfection or infection with tat-expressing vectors or HIV was observed. Equivalent levels of expression were detected after stimulation with T cell mitogens. The characteristics of 1G5 make it a valuable reagent for studies of HIV infection, HIV regulatory agents, and other T cell or HIV -activating factors, and for screening potential anti-HIV therapeutic agents.
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U2 - 10.1089/aid.1994.10.295
DO - 10.1089/aid.1994.10.295
M3 - Article
C2 - 8018390
AN - SCOPUS:0028351327
SN - 0889-2229
VL - 10
SP - 295
EP - 301
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 3
ER -