A Sensitive Reporter Cell Line for HIV-1 tat Activity, HIV-1 Inhibitors, and T Cell Activation Effects

Estuardo Aguilar-Cordova, Javier Chinen, Larry Donehower, Dorothy E. Lewis, John W. Belmont

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

The production and characterization of a cell line for quantitative HIV-1 Tat function and T cell activation assays is described. 1G5 is a clonal cell line derived from Jurkat T cells stably transfected with a luciferase gene driven by an HIV-1 long terminal repeat (HIV-LTR). The 1G5 clone was selected for low basal luciferase activity, susceptibility to HIV infection, and high responsiveness to Tat and T cell activation signals. A 10 to 1000-fold increase in luciferase activity after transfection or infection with tat-expressing vectors or HIV was observed. Equivalent levels of expression were detected after stimulation with T cell mitogens. The characteristics of 1G5 make it a valuable reagent for studies of HIV infection, HIV regulatory agents, and other T cell or HIV -activating factors, and for screening potential anti-HIV therapeutic agents.

Original languageEnglish (US)
Pages (from-to)295-301
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume10
Issue number3
DOIs
StatePublished - Mar 1994

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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