A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass

John D. Rosoff; Louise O. Soltow; Craig R. Vocelka; Gottfried Schmer; Wayne L. Chandler; R. Pat Cochran; Karyn S. Kunzelman; Bruce D. Spiess

doi:10.1016/S1053-0770(98)90191-4

A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass

John D. Rosoff, Louise O. Soltow, Craig R. Vocelka, Gottfried Schmer, Wayne L. Chandler, R. Pat Cochran, Karyn S. Kunzelman, Bruce D. Spiess

Houston Methodist

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Objectives: To examine whether a second-generation perfluorocarbon (PFC) blood substitute added to the cardiopulmonary bypass (CPB) prime influences complement production. Design: A prospective, randomized, single-blinded, ex vivo model. Setting: A university hospital, laboratory, and clinics. Participants: Ten healthy adult consented volunteer blood donors (five men, five women). Interventions: Ex vivo closed-loop extracorporeal circuit including membrane oxygenator, tubing, and filter primed with crystalloid or crystalloid plus PFC was circulated for 1 hour with the addition of 500 mL of heparinized fresh human whole blood. Measurements and Main Results: Laboratory specimens were drawn from the circuit at 10-minute intervals for 1 hour and measured for complement (C3a, Bb fragment) concentrations, blood gases, fibrinogen concentration, platelet count, and hematocrit. In the PFC group, C3a and Bb fragments were equal to or less than those in the group that received crystalloid alone. Conclusion: The second-generation PFC added to the prime of a CPB circuit does not independently increase complement production.

Original language	English (US)
Pages (from-to)	397-401
Number of pages	5
Journal	Journal of Cardiothoracic and Vascular Anesthesia
Volume	12
Issue number	4
DOIs	https://doi.org/10.1016/S1053-0770(98)90191-4
State	Published - Aug 1998

Keywords

Bb fragment
Blood substitute
C3a
Cardiopulmonary/extracorporeal bypass
Complement activation
Perfluorocarbon
Perfluorodichlorooctane

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

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10.1016/S1053-0770(98)90191-4

Cite this

Rosoff, J. D., Soltow, L. O., Vocelka, C. R., Schmer, G., Chandler, W. L., Cochran, R. P., Kunzelman, K. S., & Spiess, B. D. (1998). A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass. Journal of Cardiothoracic and Vascular Anesthesia, 12(4), 397-401. https://doi.org/10.1016/S1053-0770(98)90191-4

A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass. / Rosoff, John D.; Soltow, Louise O.; Vocelka, Craig R. et al.
In: Journal of Cardiothoracic and Vascular Anesthesia, Vol. 12, No. 4, 08.1998, p. 397-401.

Research output: Contribution to journal › Article › peer-review

Rosoff, JD, Soltow, LO, Vocelka, CR, Schmer, G, Chandler, WL, Cochran, RP, Kunzelman, KS & Spiess, BD 1998, 'A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass', Journal of Cardiothoracic and Vascular Anesthesia, vol. 12, no. 4, pp. 397-401. https://doi.org/10.1016/S1053-0770(98)90191-4

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title = "A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass",

abstract = "Objectives: To examine whether a second-generation perfluorocarbon (PFC) blood substitute added to the cardiopulmonary bypass (CPB) prime influences complement production. Design: A prospective, randomized, single-blinded, ex vivo model. Setting: A university hospital, laboratory, and clinics. Participants: Ten healthy adult consented volunteer blood donors (five men, five women). Interventions: Ex vivo closed-loop extracorporeal circuit including membrane oxygenator, tubing, and filter primed with crystalloid or crystalloid plus PFC was circulated for 1 hour with the addition of 500 mL of heparinized fresh human whole blood. Measurements and Main Results: Laboratory specimens were drawn from the circuit at 10-minute intervals for 1 hour and measured for complement (C3a, Bb fragment) concentrations, blood gases, fibrinogen concentration, platelet count, and hematocrit. In the PFC group, C3a and Bb fragments were equal to or less than those in the group that received crystalloid alone. Conclusion: The second-generation PFC added to the prime of a CPB circuit does not independently increase complement production.",

keywords = "Bb fragment, Blood substitute, C3a, Cardiopulmonary/extracorporeal bypass, Complement activation, Perfluorocarbon, Perfluorodichlorooctane",

author = "Rosoff, {John D.} and Soltow, {Louise O.} and Vocelka, {Craig R.} and Gottfried Schmer and Chandler, {Wayne L.} and Cochran, {R. Pat} and Kunzelman, {Karyn S.} and Spiess, {Bruce D.}",

note = "Funding Information: From the Departments of Anesthesiology and Surgeiy, Cardiothoracic Division, and the Department of Laboratory Medicine, University of Washington, Seattle, WA. Supported by a grant from HemaGen lnc, St Louis, MO. Presented in part at the Society of Cardiovascular Anesthesiologists' Eighteenth Annual Meeting, Salt Lake City, UT, May 6, 1996. Address reprint requests to Bruce D. Spiess, MD, University of Washington Medical Center, Department of Anesthesiology, Box 356540, 1959 NE Pacific St, Seattle, WA 98195. Copyright {\textcopyright} 1998 by WB. Saunders Company 1053-0770/98/1204-0006508.00/0 Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "1998",

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doi = "10.1016/S1053-0770(98)90191-4",

language = "English (US)",

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T1 - A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass

AU - Rosoff, John D.

AU - Soltow, Louise O.

AU - Vocelka, Craig R.

AU - Schmer, Gottfried

AU - Chandler, Wayne L.

AU - Cochran, R. Pat

AU - Kunzelman, Karyn S.

AU - Spiess, Bruce D.

N1 - Funding Information: From the Departments of Anesthesiology and Surgeiy, Cardiothoracic Division, and the Department of Laboratory Medicine, University of Washington, Seattle, WA. Supported by a grant from HemaGen lnc, St Louis, MO. Presented in part at the Society of Cardiovascular Anesthesiologists' Eighteenth Annual Meeting, Salt Lake City, UT, May 6, 1996. Address reprint requests to Bruce D. Spiess, MD, University of Washington Medical Center, Department of Anesthesiology, Box 356540, 1959 NE Pacific St, Seattle, WA 98195. Copyright © 1998 by WB. Saunders Company 1053-0770/98/1204-0006508.00/0 Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 1998/8

Y1 - 1998/8

N2 - Objectives: To examine whether a second-generation perfluorocarbon (PFC) blood substitute added to the cardiopulmonary bypass (CPB) prime influences complement production. Design: A prospective, randomized, single-blinded, ex vivo model. Setting: A university hospital, laboratory, and clinics. Participants: Ten healthy adult consented volunteer blood donors (five men, five women). Interventions: Ex vivo closed-loop extracorporeal circuit including membrane oxygenator, tubing, and filter primed with crystalloid or crystalloid plus PFC was circulated for 1 hour with the addition of 500 mL of heparinized fresh human whole blood. Measurements and Main Results: Laboratory specimens were drawn from the circuit at 10-minute intervals for 1 hour and measured for complement (C3a, Bb fragment) concentrations, blood gases, fibrinogen concentration, platelet count, and hematocrit. In the PFC group, C3a and Bb fragments were equal to or less than those in the group that received crystalloid alone. Conclusion: The second-generation PFC added to the prime of a CPB circuit does not independently increase complement production.

AB - Objectives: To examine whether a second-generation perfluorocarbon (PFC) blood substitute added to the cardiopulmonary bypass (CPB) prime influences complement production. Design: A prospective, randomized, single-blinded, ex vivo model. Setting: A university hospital, laboratory, and clinics. Participants: Ten healthy adult consented volunteer blood donors (five men, five women). Interventions: Ex vivo closed-loop extracorporeal circuit including membrane oxygenator, tubing, and filter primed with crystalloid or crystalloid plus PFC was circulated for 1 hour with the addition of 500 mL of heparinized fresh human whole blood. Measurements and Main Results: Laboratory specimens were drawn from the circuit at 10-minute intervals for 1 hour and measured for complement (C3a, Bb fragment) concentrations, blood gases, fibrinogen concentration, platelet count, and hematocrit. In the PFC group, C3a and Bb fragments were equal to or less than those in the group that received crystalloid alone. Conclusion: The second-generation PFC added to the prime of a CPB circuit does not independently increase complement production.

KW - Bb fragment

KW - Blood substitute

KW - C3a

KW - Cardiopulmonary/extracorporeal bypass

KW - Complement activation

KW - Perfluorocarbon

KW - Perfluorodichlorooctane

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A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass

Abstract

Keywords

ASJC Scopus subject areas

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