TY - JOUR
T1 - A route toward the development of 3D magnetic scaffolds with tailored mechanical and morphological properties for hard tissue regeneration
T2 - Preliminary study: A basic approach toward the design of 3D rapid prototyped magnetic scaffolds for hard-tissue regeneration is presented and validated in this paper
AU - de Santis, R.
AU - Gloria, A.
AU - Russo, T.
AU - D'Amora, U.
AU - Zeppetelli, S.
AU - Tampieri, A.
AU - Herrmannsdörfer, T.
AU - Ambrosio, L.
N1 - Funding Information:
The research leading to these results has received funding from the European Community’s 7th Framework Programme under grant agreement no. NMP3-LA-2008-214685 project MAGISTER, www.magister-project.eu.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/12
Y1 - 2011/12
N2 - A basic approach toward the design of three-dimensional (3D) rapid prototyped magnetic scaffolds for hard-tissue regeneration has been proposed. In particular, 3D scaffolds consisting of a poly(ε-caprolactone) (PCL) matrix and iron oxide (Fe 3O 4) or iron-doped hydroxyapatite (FeHA) nanoparticles were fabricated through a 3D fibre deposition technique. As a first approach, a polymer to nanoparticle weight ratio of 90/10 (wt/wt) was used. The effect of the inclusion of both kinds of nanoparticles on the mechanical, magnetic, and biological performances of the scaffolds was studied. The inclusion of Fe 3O 4 and FeHA nanoparticles generally improves the modulus and the yield stress of the fibres if compared to those of neat PCL, as well as the modulus of the scaffolds. Micro-computed tomography has confirmed the possibility to design morphologically-controlled structures with a fully interconnected pore network. Magnetisation analyses performed at 378C have highlighted M-H curves that are not hysteretic; values of saturation magnetisation (M s) of about 3.9 emu/g and 0.2 emu/g have been evaluated for PCL/Fe 3O 4 and PCL/FeHA scaffolds, respectively. Furthermore, results from confocal laser scanning microscopy (CLSM) carried out on cell-scaffold constructs have evidenced that human mesenchymal stem cells (hMSCs) better adhered and were well spread on the PCL/Fe 3O 4 and PCL/FeHA nanocomposite scaffolds in comparison with the PCL structures.
AB - A basic approach toward the design of three-dimensional (3D) rapid prototyped magnetic scaffolds for hard-tissue regeneration has been proposed. In particular, 3D scaffolds consisting of a poly(ε-caprolactone) (PCL) matrix and iron oxide (Fe 3O 4) or iron-doped hydroxyapatite (FeHA) nanoparticles were fabricated through a 3D fibre deposition technique. As a first approach, a polymer to nanoparticle weight ratio of 90/10 (wt/wt) was used. The effect of the inclusion of both kinds of nanoparticles on the mechanical, magnetic, and biological performances of the scaffolds was studied. The inclusion of Fe 3O 4 and FeHA nanoparticles generally improves the modulus and the yield stress of the fibres if compared to those of neat PCL, as well as the modulus of the scaffolds. Micro-computed tomography has confirmed the possibility to design morphologically-controlled structures with a fully interconnected pore network. Magnetisation analyses performed at 378C have highlighted M-H curves that are not hysteretic; values of saturation magnetisation (M s) of about 3.9 emu/g and 0.2 emu/g have been evaluated for PCL/Fe 3O 4 and PCL/FeHA scaffolds, respectively. Furthermore, results from confocal laser scanning microscopy (CLSM) carried out on cell-scaffold constructs have evidenced that human mesenchymal stem cells (hMSCs) better adhered and were well spread on the PCL/Fe 3O 4 and PCL/FeHA nanocomposite scaffolds in comparison with the PCL structures.
KW - Biological and mechanical analyses
KW - Hard tissue regeneration
KW - Magnetic scaffold
KW - Nanocomposite
KW - Rapid prototyping
UR - http://www.scopus.com/inward/record.url?scp=83755185896&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=83755185896&partnerID=8YFLogxK
U2 - 10.1080/17452759.2011.631324
DO - 10.1080/17452759.2011.631324
M3 - Article
AN - SCOPUS:83755185896
SN - 1745-2759
VL - 6
SP - 189
EP - 195
JO - Virtual and Physical Prototyping
JF - Virtual and Physical Prototyping
IS - 4
ER -