A route toward the development of 3D magnetic scaffolds with tailored mechanical and morphological properties for hard tissue regeneration: Preliminary study: A basic approach toward the design of 3D rapid prototyped magnetic scaffolds for hard-tissue regeneration is presented and validated in this paper

R. de Santis, A. Gloria, T. Russo, U. D'Amora, S. Zeppetelli, A. Tampieri, T. Herrmannsdörfer, L. Ambrosio

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

A basic approach toward the design of three-dimensional (3D) rapid prototyped magnetic scaffolds for hard-tissue regeneration has been proposed. In particular, 3D scaffolds consisting of a poly(ε-caprolactone) (PCL) matrix and iron oxide (Fe 3O 4) or iron-doped hydroxyapatite (FeHA) nanoparticles were fabricated through a 3D fibre deposition technique. As a first approach, a polymer to nanoparticle weight ratio of 90/10 (wt/wt) was used. The effect of the inclusion of both kinds of nanoparticles on the mechanical, magnetic, and biological performances of the scaffolds was studied. The inclusion of Fe 3O 4 and FeHA nanoparticles generally improves the modulus and the yield stress of the fibres if compared to those of neat PCL, as well as the modulus of the scaffolds. Micro-computed tomography has confirmed the possibility to design morphologically-controlled structures with a fully interconnected pore network. Magnetisation analyses performed at 378C have highlighted M-H curves that are not hysteretic; values of saturation magnetisation (M s) of about 3.9 emu/g and 0.2 emu/g have been evaluated for PCL/Fe 3O 4 and PCL/FeHA scaffolds, respectively. Furthermore, results from confocal laser scanning microscopy (CLSM) carried out on cell-scaffold constructs have evidenced that human mesenchymal stem cells (hMSCs) better adhered and were well spread on the PCL/Fe 3O 4 and PCL/FeHA nanocomposite scaffolds in comparison with the PCL structures.

Original languageEnglish (US)
Pages (from-to)189-195
Number of pages7
JournalVirtual and Physical Prototyping
Volume6
Issue number4
DOIs
StatePublished - Dec 2011

Keywords

  • Biological and mechanical analyses
  • Hard tissue regeneration
  • Magnetic scaffold
  • Nanocomposite
  • Rapid prototyping

ASJC Scopus subject areas

  • Computer Graphics and Computer-Aided Design
  • Signal Processing
  • Modeling and Simulation
  • Industrial and Manufacturing Engineering

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