A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins

Habib Ahmad, Alex Sutherland, Young Shik Shin, Kiwook Hwang, Lidong Qin, Russell John Krom, James R. Heath

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Microfluidics flow-patterning has been utilized for the construction of chip-scale miniaturized DNA and protein barcode arrays. Such arrays have been used for specific clinical and fundamental investigations in which many proteins are assayed from single cells or other small sample sizes. However, flow-patterned arrays are hand-prepared, and so are impractical for broad applications. We describe an integrated roboticsmicrofluidics platform for the automated preparation of such arrays, and we apply it to the batch fabrication of up to eighteen chips of flow-patterned DNA barcodes. The resulting substrates are comparable in quality with hand-made arrays and exhibit excellent substrate-to-substrate consistency. We demonstrate the utility and reproducibility of robotics-patterned barcodes by utilizing two flow-patterned chips for highly parallel assays of a panel of secreted proteins from single macrophage cells.

Original languageEnglish (US)
Article number094301
JournalReview of Scientific Instruments
Volume82
Issue number9
DOIs
StatePublished - Sep 2011

ASJC Scopus subject areas

  • Instrumentation

Fingerprint Dive into the research topics of 'A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins'. Together they form a unique fingerprint.

Cite this