A reversible, non-invasive method for airway resistance measurements and bronchoalveolar lavage fluid sampling in mice

Sumanth Polikepahad, Wade T. Barranco, Paul Porter, Bruce Anderson, Farrah Kheradmand, David B. Corry

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Airway hyperreactivity (AHR) measurements and bronchoalveolar lavage (BAL) fluid sampling are essential to experimental asthma models, but repeated procedures to obtain such measurements in the same animal are generally not feasible. Here, we demonstrate protocols for obtaining from mice repeated measurements of AHR and bronchoalveolar lavage fluid samples. Mice were challenged intranasally seven times over 14 days with a potent allergen or sham treated. Prior to the initial challenge, and within 24 hours following each intranasal challenge, the same animals were anesthetized, orally intubated and mechanically ventilated. AHR, assessed by comparing dose response curves of respiratory system resistance (RRS) induced by increasing intravenous doses of acetylcholine (Ach) chloride between sham and allergen-challenged animals, were determined. Afterwards, and via the same intubation, the left lung was lavaged so that differential enumeration of airway cells could be performed. These studies reveal that repeated measurements of AHR and BAL fluid collection are possible from the same animals and that maximal airway hyperresponsiveness and airway eosinophilia are achieved within 7-10 days of initiating allergen challenge. This novel technique significantly reduces the number of mice required for longitudinal experimentation and is applicable to diverse rodent species, disease models and airway physiology instruments.

Original languageEnglish
Article numbere1720
JournalJournal of Visualized Experiments
Issue number38
DOIs
StatePublished - Apr 1 2010

Keywords

  • Acetylcholine
  • Airway hyperreactivity
  • Airway resistance
  • Intubation
  • Issue 38
  • Physiology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Fingerprint Dive into the research topics of 'A reversible, non-invasive method for airway resistance measurements and bronchoalveolar lavage fluid sampling in mice'. Together they form a unique fingerprint.

Cite this