A rapid method for obtaining mesenchymal stem cells and platelets from bone marrow aspirate

Barbara Dozza, Giuliana Gobbi, Enrico Lucarelli, Michela Pierini, Claudia Di Bella, Tommaso Frisoni, Pier Luigi Tazzari, Francesca Ricci, Prisco Mirandola, Cecilia Carubbi, Sandro Giannini, Davide Donati, Marco Vitale

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) are currently used alone or in combination for therapeutic applications especially for bone repair. We tested whether MSCs can be isolated from bone marrow (BM) aspirate using a commercially available kit commonly used to obtain PRP from peripheral blood (PB). Results revealed that mononuclear cells and platelets from both PB and BM could be efficiently isolated by obtaining a mononuclear and platelet rich fraction (PB-MPRF and BM-MPRF, respectively). Starting with comparable volumes, the number of platelets increased 1.5-fold in BM-MPRF compared to PB-MPRF. The number of clonogenic cells in BM-MPRF samples was significantly higher than whole BM samples as revealed by CFU-F assay (54.92±8.55CFU-F/1.5 x 105 nucleated cells and 32.50±12.43CFU-F/1.5 x 105 nucleated cells, respectively). Cells isolated from BM-MPRF after in vitro expansion fulfilled the definition of MSCs by phenotypic criteria, and differentiated along osteogenic, adipogenic and chondrogenic lineages following induction. Results showed that the kit isolated MSCs and platelets from BM aspirate. Isolated MSCs were further expanded in a laboratory and BM-MPRF was used clinically following BM withdrawal for rapid intra-operative cell therapy for the treatment of bone defects.

Original languageEnglish (US)
Pages (from-to)483-492
Number of pages10
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume8
Issue number6
DOIs
StatePublished - Jun 1 2014

Keywords

  • Bone marrow aspirate
  • Mesenchymal stem cells
  • Osteocompetent cells
  • Platelet-rich plasma
  • Platelets

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Medicine(all)

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