A Plaque Method for Titration of Frog Viruses Using Starch Gel Overlay

Daniel E. Lehane, H. Fred Clark, David T. Karzon

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A reliable plaque assay system has been developed for several recently isolated frog viruses. Cell lines from reptiles, amphibians, and fish were tested for their ability to be maintained under various solid overlay materials. Starch gel was found to be the most useful overlay material. The most sensitive assay system was shown to be Terrapene heart (TH-1) cell culture.

Original languageEnglish (US)
Pages (from-to)50-54
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume125
Issue number1
DOIs
StatePublished - May 1967

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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