A peroxidase-active aptazyme as an isothermally amplifiable label in an aptazyme-linked oligonucleotide assay for low-picomolar IgE detection

Jeroen Pollet; Ulrich Strych; Richard C. Willson

doi:10.1039/c2an36201e

A peroxidase-active aptazyme as an isothermally amplifiable label in an aptazyme-linked oligonucleotide assay for low-picomolar IgE detection

Jeroen Pollet, Ulrich Strych, Richard C. Willson

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A peroxidase-active hemin-aptamer was clicked onto an immunoglobulin E (IgE) binding aptamer. The resulting bifunctional aptamer was successfully used in a highly sensitive aptazyme-linked oligonucleotide assay (ALONA) for the detection of IgE. The sensitivity of the assay is enhanced by isothermal amplification of the aptazyme followed by colorimetric detection of the aptazyme's peroxidase activity, which can easily be evaluated even without advanced lab instruments. This journal is

Original language	English (US)
Pages (from-to)	5710-5712
Number of pages	3
Journal	Analyst
Volume	137
Issue number	24
DOIs	https://doi.org/10.1039/c2an36201e
State	Published - Dec 21 2012

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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A peroxidase-active aptazyme as an isothermally amplifiable label in an aptazyme-linked oligonucleotide assay for low-picomolar IgE detection

Abstract

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