A novel virus-encoded nucleocytoplasmic shuttling protein: The UL3 protein of herpes simplex virus type 1

Chunfu Zheng, Fusen Lin, Shuai Wang, Junji Xing

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.

Original languageEnglish (US)
Pages (from-to)206-210
Number of pages5
JournalJournal of Virological Methods
Volume177
Issue number2
DOIs
StatePublished - Nov 2011

Keywords

  • Chromosomal region maintenance 1
  • HSV-1
  • Nuclear export signal
  • Nuclear localization signal
  • Ran-GTP
  • UL3

ASJC Scopus subject areas

  • Virology

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