TY - JOUR
T1 - A novel virus-encoded nucleocytoplasmic shuttling protein
T2 - The UL3 protein of herpes simplex virus type 1
AU - Zheng, Chunfu
AU - Lin, Fusen
AU - Wang, Shuai
AU - Xing, Junji
PY - 2011/11
Y1 - 2011/11
N2 - Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.
AB - Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.
KW - Chromosomal region maintenance 1
KW - HSV-1
KW - Nuclear export signal
KW - Nuclear localization signal
KW - Ran-GTP
KW - UL3
UR - http://www.scopus.com/inward/record.url?scp=80053131428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053131428&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2011.08.004
DO - 10.1016/j.jviromet.2011.08.004
M3 - Article
C2 - 21864580
AN - SCOPUS:80053131428
VL - 177
SP - 206
EP - 210
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 2
ER -