A novel virus-encoded nucleocytoplasmic shuttling protein: The UL3 protein of herpes simplex virus type 1

Chunfu Zheng; Fusen Lin; Shuai Wang; Junji Xing

doi:10.1016/j.jviromet.2011.08.004

A novel virus-encoded nucleocytoplasmic shuttling protein: The UL3 protein of herpes simplex virus type 1

Chunfu Zheng, Fusen Lin, Shuai Wang, Junji Xing

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.

Original language	English (US)
Pages (from-to)	206-210
Number of pages	5
Journal	Journal of Virological Methods
Volume	177
Issue number	2
DOIs	https://doi.org/10.1016/j.jviromet.2011.08.004
State	Published - Nov 2011

Keywords

Chromosomal region maintenance 1
HSV-1
Nuclear export signal
Nuclear localization signal
Ran-GTP
UL3

ASJC Scopus subject areas

Virology

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10.1016/j.jviromet.2011.08.004

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title = "A novel virus-encoded nucleocytoplasmic shuttling protein: The UL3 protein of herpes simplex virus type 1",

abstract = "Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.",

keywords = "Chromosomal region maintenance 1, HSV-1, Nuclear export signal, Nuclear localization signal, Ran-GTP, UL3",

author = "Chunfu Zheng and Fusen Lin and Shuai Wang and Junji Xing",

note = "Funding Information: We are grateful to Dr. Yoshinari Yasuda for providing pGEX-6p-1 Q69L Ran and Dr. Gillian Elliott for the generous gift plasmid pRev-NES-EGFP. This research was supported by the Major State Basic Research Development Program of China (973 Program) ( 2010CB530105 and 2011CB504802 ); the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences ( 20071010-141 ) and the National Natural Science Foundation of China ( 30870120 , 30900059 and 81000736 ). Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2011",

month = nov,

doi = "10.1016/j.jviromet.2011.08.004",

language = "English (US)",

volume = "177",

pages = "206--210",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "2",

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T1 - A novel virus-encoded nucleocytoplasmic shuttling protein

T2 - The UL3 protein of herpes simplex virus type 1

AU - Zheng, Chunfu

AU - Lin, Fusen

AU - Wang, Shuai

AU - Xing, Junji

N1 - Funding Information: We are grateful to Dr. Yoshinari Yasuda for providing pGEX-6p-1 Q69L Ran and Dr. Gillian Elliott for the generous gift plasmid pRev-NES-EGFP. This research was supported by the Major State Basic Research Development Program of China (973 Program) ( 2010CB530105 and 2011CB504802 ); the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences ( 20071010-141 ) and the National Natural Science Foundation of China ( 30870120 , 30900059 and 81000736 ). Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2011/11

Y1 - 2011/11

N2 - Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.

AB - Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.

KW - Chromosomal region maintenance 1

KW - HSV-1

KW - Nuclear export signal

KW - Nuclear localization signal

KW - Ran-GTP

KW - UL3

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A novel virus-encoded nucleocytoplasmic shuttling protein: The UL3 protein of herpes simplex virus type 1

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Keywords

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