A novel transgenic marker for migrating limb muscle precursors and for vascular smooth muscle cells

Avital Tidhar, Moshe Reichenstein, Dana Cohen, Alexander Faerman, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Moshe Shani

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

A unique pattern of LacZ expression was found in a transgenic mouse line, likely due to regulatory elements at the site of integration. Two new genes flanking the transgene were identified. At early stages of development, the transgene is transiently expressed in ventro-lateral demomyotomal cells migrating from the somites into the limb buds. At late developmental stages and in the adult, lacZ staining marks vascular smooth muscle cells throughout the vascular bed, with the exception of the major elastic arteries, and in pericytes. No expression was detected in skeletal and smooth muscles. Different patterns of expression in vascular smooth muscles was observed at distinct levels of the vascular tree, in arteries as well as in veins. Vessel injury, resulting in stimulation of smooth muscle cells proliferation and migration, is associated with transgene down-regulation. After the formation of neointima thickening, it is reactivated. This transgenic insertion may therefore be used as a useful marker to identify novel physiological cues or genetic elements involved in the regulation of the vascular smooth muscle phenotype(s). It may also provide an experimental tool for studying vasculature and the involvement of pericytes in regulating microvascular homeostasis.

Original languageEnglish (US)
Pages (from-to)60-73
Number of pages14
JournalDevelopmental Dynamics
Volume220
Issue number1
DOIs
StatePublished - 2001

Keywords

  • Cell migration
  • Insertional mutagenesis
  • Limb muscles
  • Neointima
  • Pericytes
  • Transgenic mice
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'A novel transgenic marker for migrating limb muscle precursors and for vascular smooth muscle cells'. Together they form a unique fingerprint.

Cite this