A novel MSCRAMM subfamily in coagulase negative staphylococcal species

Srishtee Arora, Anne Catrin Uhlemann, Franklin D. Lowy, Magnus Hook

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Coagulase negative staphylococci (CoNS) are important opportunistic pathogens. Staphylococcus epidermidis, a coagulase negative staphylococcus, is the third leading cause of nosocomial infections in the US. Surface proteins like Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) are major virulence factors of pathogenic gram positive bacteria. Here, we identified a new chimeric protein in S. epidermidis, that we call SesJ. SesJ represents a prototype of a new subfamily of MSCRAMMs. Structural predictions show that SesJ has structural features characteristic of a MSCRAMM along with a N-terminal repeat region and an aspartic acid containing C-terminal repeat region, features that have not been previously observed in staphylococcal MSCRAMMs but have been found in other surface proteins from gram positive bacteria. We identified and analyzed structural homologs of SesJ in three other CoNS. These homologs of SesJ have an identical structural organization but varying sequence identities within the domains. Using flow cytometry, we also show that SesJ is expressed constitutively on the surface of a representative S. epidermidis strain, from early exponential to stationary growth phase. Thus, SesJ is positioned to interact with protein targets in the environment and plays a role in S. epidermidis virulence.

Original languageEnglish (US)
Article number540
JournalFrontiers in Microbiology
Issue numberAPR
StatePublished - Apr 29 2016


  • Cell wall anchored proteins
  • Coagulase negative staphylococci
  • N-terminal repeats
  • Staphylococcus epidermidis
  • Structural homologs

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)


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