TY - JOUR
T1 - A novel method to detect Listeria monocytogenes via superparamagnetic lateral flow immunoassay
AU - Shi, Lei
AU - Wu, Feng
AU - Wen, Yiming
AU - Zhao, Fang
AU - Xiang, Junjian
AU - Ma, Lan
N1 - Publisher Copyright:
© 2014 Springer-Verlag Berlin Heidelberg.
PY - 2015/1/21
Y1 - 2015/1/21
N2 - A novel strip test system combining immunomagnetic separation with lateral flow immunoassay (LFIA) was established for the accurate detection of Listeria monocytogenes. In this system, a pair of matched monoclonal antibodies was used to construct a sandwich immunoassay, in which superparamagnetic particles were coupled with one of the antibodies as a labeled antibody to capture the target bacteria, while the other antibody was immobilized on the detection zone. After a 20-min reaction, the strips were analyzed by a novel instrument which could detect the magnetic signal of the immunocomplex in a magnetic field. Sensitivity evaluation showed that the limit of detection (LOD) of the superparamagnetic LFIA system for L. monocytogenes was 104 CFU/mL, which was at least one log lower than conventional LFIA. No cross-reaction was observed when Salmonella, Escherichia coli O157:H7, or three types of harmless Listeria strains were tested. Further evaluation with actual food samples indicated that the superparamagnetic LFIA system showed 100 % concordance with real-time PCR. Therefore, this novel superparamagnetic LFIA system could be used as a rapid, sensitive, and specific method for the detection of L. monocytogenes. [Figure not available: see fulltext.]
AB - A novel strip test system combining immunomagnetic separation with lateral flow immunoassay (LFIA) was established for the accurate detection of Listeria monocytogenes. In this system, a pair of matched monoclonal antibodies was used to construct a sandwich immunoassay, in which superparamagnetic particles were coupled with one of the antibodies as a labeled antibody to capture the target bacteria, while the other antibody was immobilized on the detection zone. After a 20-min reaction, the strips were analyzed by a novel instrument which could detect the magnetic signal of the immunocomplex in a magnetic field. Sensitivity evaluation showed that the limit of detection (LOD) of the superparamagnetic LFIA system for L. monocytogenes was 104 CFU/mL, which was at least one log lower than conventional LFIA. No cross-reaction was observed when Salmonella, Escherichia coli O157:H7, or three types of harmless Listeria strains were tested. Further evaluation with actual food samples indicated that the superparamagnetic LFIA system showed 100 % concordance with real-time PCR. Therefore, this novel superparamagnetic LFIA system could be used as a rapid, sensitive, and specific method for the detection of L. monocytogenes. [Figure not available: see fulltext.]
KW - Lateral flow immunoassay
KW - Listeria monocytogenes
KW - Sandwich immunoassay
KW - Superparamagnetic
UR - http://www.scopus.com/inward/record.url?scp=84930670610&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84930670610&partnerID=8YFLogxK
U2 - 10.1007/s00216-014-8276-8
DO - 10.1007/s00216-014-8276-8
M3 - Article
C2 - 25486917
AN - SCOPUS:84930670610
SN - 1618-2642
VL - 407
SP - 529
EP - 535
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 2
ER -