Abstract
cDNA clones corresponding to an Mr ∼ 80 000 receptor (type I receptor) for interleukin-1 (IL-1) have been isolated previously by mammalian expression. Here, we report the use of an improved expression cloning method to isolate human and murine cDNA clones encoding a second type (Mr ∼ 60 000) of IL-1 receptor (type II receptor). The mature type II IL-1 receptor consists of (i) a ligand binding portion comprised of three immunoglobulin-like domains; (ii) a single transmembrane region; and (iii) a short cytoplasmic domain of 29 amino acids. This last contrasts with the ∼ 215 amino acid cytoplasmic domain of the type I receptor, and suggests that the two IL-1 receptors may interact with different signal transduction pathways. The type II receptor is expressed in a number of different tissues, including both B and T lymphocytes, and can be induced in several cell types by treatment with phorbol ester. Both IL-1 receptors appear to be well conserved in evolution, and map to the same chromosomal location. Like the type I receptor, the human type II IL-1 receptor can bind all three forms of IL-1 (IL-1α, IL-1β and IL-1ra). Vaccinia virus contains an open reading frame bearing strong resemblance to the type II IL-1 receptor.
Original language | English (US) |
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Pages (from-to) | 2821-2832 |
Number of pages | 12 |
Journal | EMBO Journal |
Volume | 10 |
Issue number | 10 |
DOIs | |
State | Published - 1991 |
Keywords
- B cell
- Chromosome mapping
- Expression cloning
- Interleukin-1
- Receptor
ASJC Scopus subject areas
- Cell Biology
- Genetics