A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types

Catherine J. McMahan, Jennifer L. Slack, Bruce Mosley, David Cosman, Stephen D. Lupton, Laurie L. Brunton, Catherine E. Grubin, Janis M. Wignall, Nancy A. Jenkins, Camilynn I. Brannan, Neal G. Copeland, Kay Huebner, Carlo M. Croce, Linda A. Cannizzarro, David Benjamin, Steven K. Dower, Melanie K. Spriggs, John E. Sims

Research output: Contribution to journalArticlepeer-review

672 Scopus citations


cDNA clones corresponding to an Mr ∼ 80 000 receptor (type I receptor) for interleukin-1 (IL-1) have been isolated previously by mammalian expression. Here, we report the use of an improved expression cloning method to isolate human and murine cDNA clones encoding a second type (Mr ∼ 60 000) of IL-1 receptor (type II receptor). The mature type II IL-1 receptor consists of (i) a ligand binding portion comprised of three immunoglobulin-like domains; (ii) a single transmembrane region; and (iii) a short cytoplasmic domain of 29 amino acids. This last contrasts with the ∼ 215 amino acid cytoplasmic domain of the type I receptor, and suggests that the two IL-1 receptors may interact with different signal transduction pathways. The type II receptor is expressed in a number of different tissues, including both B and T lymphocytes, and can be induced in several cell types by treatment with phorbol ester. Both IL-1 receptors appear to be well conserved in evolution, and map to the same chromosomal location. Like the type I receptor, the human type II IL-1 receptor can bind all three forms of IL-1 (IL-1α, IL-1β and IL-1ra). Vaccinia virus contains an open reading frame bearing strong resemblance to the type II IL-1 receptor.

Original languageEnglish (US)
Pages (from-to)2821-2832
Number of pages12
JournalEMBO Journal
Issue number10
StatePublished - 1991


  • B cell
  • Chromosome mapping
  • Expression cloning
  • Interleukin-1
  • Receptor

ASJC Scopus subject areas

  • Cell Biology
  • Genetics


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