A newly identified enhancer element responsible for type II collagen gene expression

Tamayuki Shinomura, Kazuo Ito, Magnus Höök, James H. Kimura

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Type II collagen is a major component of cartilage where it is present at a high concentration, which is essential for the functional maintenance of the tissue. Therefore, any fundamental understanding of the physiology of cartilage tissue must include an understanding of the mechanism that allows the high level of expression of type II collagen gene, Col2a1, by chondrocytes. To this end, we developed a new reporter assay system based on the co-transfection of candidate enhancer elements and reporter construct into Swarm rat chondrosarcoma chondrocytes that allowed their stable expression. Using this system, we screened more than 70 kb of the Col2a1 gene and found an enhancer domain that is responsible for the regulation of its expression level. The domain is localized in intron 7, and consists of an 800-bp region that contains within it a previously unidentified domain, ∼140 bp in size.

Original languageEnglish (US)
Pages (from-to)565-575
Number of pages11
JournalJournal of Biochemistry
Issue number6
StatePublished - Dec 2012


  • chondrocytes
  • enhancer
  • extracellular matrix
  • gene expression
  • type II collagen

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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