A new imaging platform for visualizing biological effects of non-invasive radiofrequency electric-field cancer hyperthermia

Stuart J. Corr, Sabeel Shamsudeen, Leoncio A. Vergara, Jason Chak-Shing Ho, Matthew J. Ware, Vazrik Keshishian, Kenji Yokoi, David J. Savage, Ismail M. Meraz, Warna Kaluarachchi, Brandon T. Cisneros, Mustafa Raoof, Duy Trac Nguyen, Yingchun Zhang, Lon J. Wilson, Huw Summers, Paul Rees, Steven A. Curley, Rita E. Serda

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel structure and drug perfusion. Our results indicate that the IVM system is able to handle exposure to high-power electric-fields without inducing significant hardware damage or imaging artifacts. Furthermore, short durations of low-power (< 200 W) radiofrequency exposure increased transport and perfusion of fluorescent tracers into the tumors at temperatures below 41°C. Vessel deformations and blood coagulation were seen for tumor temperatures around 44°C. These results highlight the use of our integrated IVM-RF imaging platform as a powerful new tool to visualize the dynamics and interplay between radiofrequency energy and biological tissues, organs, and tumors.

Original languageEnglish (US)
Article numbere0136382
JournalPLoS ONE
Volume10
Issue number8
DOIs
StatePublished - Aug 26 2015

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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