A new imaging platform for visualizing biological effects of non-invasive radiofrequency electric-field cancer hyperthermia

Stuart J. Corr; Sabeel Shamsudeen; Leoncio A. Vergara; Jason Chak-Shing Ho; Matthew J. Ware; Vazrik Keshishian; Kenji Yokoi; David J. Savage; Ismail M. Meraz; Warna Kaluarachchi; Brandon T. Cisneros; Mustafa Raoof; Duy Trac Nguyen; Yingchun Zhang; Lon J. Wilson; Huw Summers; Paul Rees; Steven A. Curley; Rita E. Serda

doi:10.1371/journal.pone.0136382

A new imaging platform for visualizing biological effects of non-invasive radiofrequency electric-field cancer hyperthermia

Stuart J. Corr, Sabeel Shamsudeen, Leoncio A. Vergara, Jason Chak-Shing Ho, Matthew J. Ware, Vazrik Keshishian, Kenji Yokoi, David J. Savage, Ismail M. Meraz, Warna Kaluarachchi, Brandon T. Cisneros, Mustafa Raoof, Duy Trac Nguyen, Yingchun Zhang, Lon J. Wilson, Huw Summers, Paul Rees, Steven A. Curley, Rita E. Serda

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Abstract

Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel structure and drug perfusion. Our results indicate that the IVM system is able to handle exposure to high-power electric-fields without inducing significant hardware damage or imaging artifacts. Furthermore, short durations of low-power (< 200 W) radiofrequency exposure increased transport and perfusion of fluorescent tracers into the tumors at temperatures below 41°C. Vessel deformations and blood coagulation were seen for tumor temperatures around 44°C. These results highlight the use of our integrated IVM-RF imaging platform as a powerful new tool to visualize the dynamics and interplay between radiofrequency energy and biological tissues, organs, and tumors.

Original language	English (US)
Article number	e0136382
Journal	PLoS ONE
Volume	10
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0136382
State	Published - Aug 26 2015

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Corr, S. J., Shamsudeen, S., Vergara, L. A., Chak-Shing Ho, J., Ware, M. J., Keshishian, V., Yokoi, K., Savage, D. J., Meraz, I. M., Kaluarachchi, W., Cisneros, B. T., Raoof, M., Nguyen, D. T., Zhang, Y., Wilson, L. J., Summers, H., Rees, P., Curley, S. A., & Serda, R. E. (2015). A new imaging platform for visualizing biological effects of non-invasive radiofrequency electric-field cancer hyperthermia. PLoS ONE, 10(8), Article e0136382. https://doi.org/10.1371/journal.pone.0136382

Corr, SJ, Shamsudeen, S, Vergara, LA, Chak-Shing Ho, J, Ware, MJ, Keshishian, V, Yokoi, K, Savage, DJ, Meraz, IM, Kaluarachchi, W, Cisneros, BT, Raoof, M, Nguyen, DT, Zhang, Y, Wilson, LJ, Summers, H, Rees, P, Curley, SA & Serda, RE 2015, 'A new imaging platform for visualizing biological effects of non-invasive radiofrequency electric-field cancer hyperthermia', PLoS ONE, vol. 10, no. 8, e0136382. https://doi.org/10.1371/journal.pone.0136382

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title = "A new imaging platform for visualizing biological effects of non-invasive radiofrequency electric-field cancer hyperthermia",

abstract = "Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel structure and drug perfusion. Our results indicate that the IVM system is able to handle exposure to high-power electric-fields without inducing significant hardware damage or imaging artifacts. Furthermore, short durations of low-power (< 200 W) radiofrequency exposure increased transport and perfusion of fluorescent tracers into the tumors at temperatures below 41°C. Vessel deformations and blood coagulation were seen for tumor temperatures around 44°C. These results highlight the use of our integrated IVM-RF imaging platform as a powerful new tool to visualize the dynamics and interplay between radiofrequency energy and biological tissues, organs, and tumors.",

author = "Corr, {Stuart J.} and Sabeel Shamsudeen and Vergara, {Leoncio A.} and {Chak-Shing Ho}, Jason and Ware, {Matthew J.} and Vazrik Keshishian and Kenji Yokoi and Savage, {David J.} and Meraz, {Ismail M.} and Warna Kaluarachchi and Cisneros, {Brandon T.} and Mustafa Raoof and Nguyen, {Duy Trac} and Yingchun Zhang and Wilson, {Lon J.} and Huw Summers and Paul Rees and Curley, {Steven A.} and Serda, {Rita E.}",

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AU - Chak-Shing Ho, Jason

AU - Ware, Matthew J.

AU - Keshishian, Vazrik

AU - Yokoi, Kenji

AU - Savage, David J.

AU - Meraz, Ismail M.

AU - Kaluarachchi, Warna

AU - Cisneros, Brandon T.

AU - Raoof, Mustafa

AU - Nguyen, Duy Trac

AU - Zhang, Yingchun

AU - Wilson, Lon J.

AU - Summers, Huw

AU - Rees, Paul

AU - Curley, Steven A.

AU - Serda, Rita E.

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AB - Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel structure and drug perfusion. Our results indicate that the IVM system is able to handle exposure to high-power electric-fields without inducing significant hardware damage or imaging artifacts. Furthermore, short durations of low-power (< 200 W) radiofrequency exposure increased transport and perfusion of fluorescent tracers into the tumors at temperatures below 41°C. Vessel deformations and blood coagulation were seen for tumor temperatures around 44°C. These results highlight the use of our integrated IVM-RF imaging platform as a powerful new tool to visualize the dynamics and interplay between radiofrequency energy and biological tissues, organs, and tumors.

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A new imaging platform for visualizing biological effects of non-invasive radiofrequency electric-field cancer hyperthermia

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