Abstract
A new procedure for the detection and quantitation of organic compounds in thin-layer chromatography is described in which the formation of fluorescent derivatives is induced by thermal treatment of the chromatoplates in the presence of ammonium hydrogen carbonate, and the fluorophores formed are quantitated on the plate by spectrofluorimetry. By using the detecting agent in the vapor phase, a complete, uniform reaction and a regular background are obtained. This procedure offers the advantages of convenience, sensitivity, versatility, stability of the fluorophores and of using an inexpensive, non-toxic reagent that is easy to handle.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 643-657 |
| Number of pages | 15 |
| Journal | Journal of Chromatography A |
| Volume | 99 |
| Issue number | C |
| DOIs | |
| State | Published - Nov 6 1974 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
Fingerprint
Dive into the research topics of 'A new fluorimetric procedure for the detection and quantitation of organic compounds in thin-layer chromatography'. Together they form a unique fingerprint.Cite this
- APA
- Standard
- Harvard
- Vancouver
- Author
- BIBTEX
- RIS