A new fluorimetric procedure for the detection and quantitation of organic compounds in thin-layer chromatography

Ramon Segura, Antonio M. Gotto

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

A new procedure for the detection and quantitation of organic compounds in thin-layer chromatography is described in which the formation of fluorescent derivatives is induced by thermal treatment of the chromatoplates in the presence of ammonium hydrogen carbonate, and the fluorophores formed are quantitated on the plate by spectrofluorimetry. By using the detecting agent in the vapor phase, a complete, uniform reaction and a regular background are obtained. This procedure offers the advantages of convenience, sensitivity, versatility, stability of the fluorophores and of using an inexpensive, non-toxic reagent that is easy to handle.

Original languageEnglish (US)
Pages (from-to)643-657
Number of pages15
JournalJournal of Chromatography A
Volume99
Issue numberC
DOIs
StatePublished - Nov 6 1974

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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