TY - JOUR
T1 - A new fluorimetric procedure for the detection and quantitation of organic compounds in thin-layer chromatography
AU - Segura, Ramon
AU - Gotto, Antonio M.
N1 - Funding Information:
This work was supported in part by contract NIH-NHLI-71-2156 from the National Heart and Lung Institute.
PY - 1974/11/6
Y1 - 1974/11/6
N2 - A new procedure for the detection and quantitation of organic compounds in thin-layer chromatography is described in which the formation of fluorescent derivatives is induced by thermal treatment of the chromatoplates in the presence of ammonium hydrogen carbonate, and the fluorophores formed are quantitated on the plate by spectrofluorimetry. By using the detecting agent in the vapor phase, a complete, uniform reaction and a regular background are obtained. This procedure offers the advantages of convenience, sensitivity, versatility, stability of the fluorophores and of using an inexpensive, non-toxic reagent that is easy to handle.
AB - A new procedure for the detection and quantitation of organic compounds in thin-layer chromatography is described in which the formation of fluorescent derivatives is induced by thermal treatment of the chromatoplates in the presence of ammonium hydrogen carbonate, and the fluorophores formed are quantitated on the plate by spectrofluorimetry. By using the detecting agent in the vapor phase, a complete, uniform reaction and a regular background are obtained. This procedure offers the advantages of convenience, sensitivity, versatility, stability of the fluorophores and of using an inexpensive, non-toxic reagent that is easy to handle.
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U2 - 10.1016/S0021-9673(00)90892-6
DO - 10.1016/S0021-9673(00)90892-6
M3 - Article
C2 - 4371773
AN - SCOPUS:0016139524
SN - 0021-9673
VL - 99
SP - 643
EP - 657
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -