A murine homolog of the yeast RNA1 gene is required for postimplantation development

James DeGregori, Andreas Russ, Harald Von Melchner, Helen Rayburn, Prashant Priyaranjan, Nancy A. Jenkins, Neal G. Copeland, H. Earl Ruley

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

A gene has been characterized that is required for postimplantation mouse development. The gene, designated fug1, was disrupted in embryonic stem cells by the U3Neo gene trap retrovirus, and the disrupted allele was introduced into the germ line. Homozygous mutant embryos arrest at the egg cylinder stage at about embryonic day 6 and are mostly resorbed by day 8.5. The appearance of the proamniotic cavity is delayed, and epiblast cells that surround the cavity are disorganized. fug1 transcripts are undetectable at E6 but are induced throughout the embryo after E6.5. The gene is expressed at low levels in all adult tissues examined, maps to chromosome 15, and is conserved among mammals. The cDNA sequence encodes a protein of 589 amino acids, the first 400 of which are 38% identical to the Saccharomyces cerevisiae RNA1 gene. Regions of greatest similarity include a long acidic domain and 11 leucine-rich motifs, thought to mediate high affinity protein- protein interactions. These similarities suggest that Fug1 may be required for developmental changes in RNA processing or chromatin structure prior to gastrulation.

Original languageEnglish (US)
Pages (from-to)265-276
Number of pages12
JournalGenes and Development
Volume8
Issue number3
DOIs
StatePublished - 1994

Keywords

  • fug1
  • insertional mutagenesis
  • leucine rich motifs
  • mouse development
  • yeast RNA1

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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