A modified method for the simultaneous determination of regional single-transit brain extraction of diffusion-limited compounds and cerebral blood flow: Utilization of non-invasive measurement of transit time

Thomas A. Kent; Sheldon H. Preskorn

doi:10.1016/0006-8993(85)90477-9

A modified method for the simultaneous determination of regional single-transit brain extraction of diffusion-limited compounds and cerebral blood flow: Utilization of non-invasive measurement of transit time

Thomas A. Kent, Sheldon H. Preskorn

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A dual-label radioisotope method to measure regional cerebral blood flow (CBF) with [¹⁴C]butanol and the single-transit brain extraction of [³H]water (E_w) was modified to permit concomittant measurement of the exit time through the cerebrovasculature of a bolus (BET) of ⁵¹chromium (⁵¹Cr)-labeled ethylenediaminetetraacetic acid (EDTA) in rodents. First, [⁵¹Cr]EDTA was injected intravenously via the femoral vein and its transit through cerebrovasculature determined by external gamma counting. The BET measurement was then used to determine the optimum time for animal sacrifice for subsequent measurement of CBF and E_w to minimize both intravascular contamination and washout of butanol and water. This procedure resulted in higher CBF at moderate hypercapnia and slightly lower E_w as a function of arterial CO₂ content than previously found using a fixed interval for sacrifice.

Original language	English (US)
Pages (from-to)	251-255
Number of pages	5
Journal	Brain Research
Volume	335
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(85)90477-9
State	Published - Jun 3 1985

Keywords

CO responsivity
blood-brain barrier
cerebral blood flow
cerebral blood volume
cerebral transit time
extraction fraction

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(85)90477-9

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A modified method for the simultaneous determination of regional single-transit brain extraction of diffusion-limited compounds and cerebral blood flow : Utilization of non-invasive measurement of transit time. / Kent, Thomas A.; Preskorn, Sheldon H.

In: Brain Research, Vol. 335, No. 2, 03.06.1985, p. 251-255.

Research output: Contribution to journal › Article › peer-review

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title = "A modified method for the simultaneous determination of regional single-transit brain extraction of diffusion-limited compounds and cerebral blood flow: Utilization of non-invasive measurement of transit time",

abstract = "A dual-label radioisotope method to measure regional cerebral blood flow (CBF) with [14C]butanol and the single-transit brain extraction of [3H]water (Ew) was modified to permit concomittant measurement of the exit time through the cerebrovasculature of a bolus (BET) of 51chromium (51Cr)-labeled ethylenediaminetetraacetic acid (EDTA) in rodents. First, [51Cr]EDTA was injected intravenously via the femoral vein and its transit through cerebrovasculature determined by external gamma counting. The BET measurement was then used to determine the optimum time for animal sacrifice for subsequent measurement of CBF and Ew to minimize both intravascular contamination and washout of butanol and water. This procedure resulted in higher CBF at moderate hypercapnia and slightly lower Ew as a function of arterial CO2 content than previously found using a fixed interval for sacrifice.",

keywords = "CO responsivity, blood-brain barrier, cerebral blood flow, cerebral blood volume, cerebral transit time, extraction fraction",

author = "Kent, {Thomas A.} and Preskorn, {Sheldon H.}",

note = "Funding Information: The authors thank Dr. Ralph N. Adams, Dr. Greg A. Gerhardt, Rick Yang, Charles Bradberry and Margaret Rice for assistance with the electronics and Betty Stone and Adelaide Lumary for manuscript preparation. This work is supported in part by a Clinical Pharmacology Fellowship from the Pharmaceut- Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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N2 - A dual-label radioisotope method to measure regional cerebral blood flow (CBF) with [14C]butanol and the single-transit brain extraction of [3H]water (Ew) was modified to permit concomittant measurement of the exit time through the cerebrovasculature of a bolus (BET) of 51chromium (51Cr)-labeled ethylenediaminetetraacetic acid (EDTA) in rodents. First, [51Cr]EDTA was injected intravenously via the femoral vein and its transit through cerebrovasculature determined by external gamma counting. The BET measurement was then used to determine the optimum time for animal sacrifice for subsequent measurement of CBF and Ew to minimize both intravascular contamination and washout of butanol and water. This procedure resulted in higher CBF at moderate hypercapnia and slightly lower Ew as a function of arterial CO2 content than previously found using a fixed interval for sacrifice.

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KW - extraction fraction

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A modified method for the simultaneous determination of regional single-transit brain extraction of diffusion-limited compounds and cerebral blood flow: Utilization of non-invasive measurement of transit time

Abstract

Keywords

ASJC Scopus subject areas

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