A method to extract intact and pure RNA from mycobacteria

Shamim Akhtar; Sampa Sarkar; Abhishek Mishra; Dhiman Sarkar

doi:10.1016/j.ab.2011.06.028

A method to extract intact and pure RNA from mycobacteria

Shamim Akhtar, Sampa Sarkar, Abhishek Mishra, Dhiman Sarkar

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9 Scopus citations

Abstract

We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 μg of RNA from 10⁷ cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages.

Original language	English (US)
Pages (from-to)	286-288
Number of pages	3
Journal	Analytical Biochemistry
Volume	417
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2011.06.028
State	Published - Oct 15 2011

Keywords

Dormant
Hypoxia
Mycobacteria
RNA isolation
Spheroplast
Trizol

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2011.06.028

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abstract = "We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 μg of RNA from 107 cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages.",

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author = "Shamim Akhtar and Sampa Sarkar and Abhishek Mishra and Dhiman Sarkar",

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AU - Akhtar, Shamim

AU - Sarkar, Sampa

AU - Mishra, Abhishek

AU - Sarkar, Dhiman

PY - 2011/10/15

Y1 - 2011/10/15

N2 - We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 μg of RNA from 107 cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages.

AB - We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 μg of RNA from 107 cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages.

KW - Dormant

KW - Hypoxia

KW - Mycobacteria

KW - RNA isolation

KW - Spheroplast

KW - Trizol

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

A method to extract intact and pure RNA from mycobacteria

Abstract

Keywords

ASJC Scopus subject areas

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