A method to extract intact and pure RNA from mycobacteria

Shamim Akhtar, Sampa Sarkar, Abhishek Mishra, Dhiman Sarkar

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 μg of RNA from 107 cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages.

Original languageEnglish (US)
Pages (from-to)286-288
Number of pages3
JournalAnalytical Biochemistry
Issue number2
StatePublished - Oct 15 2011


  • Dormant
  • Hypoxia
  • Mycobacteria
  • RNA isolation
  • Spheroplast
  • Trizol

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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