A membrane-based force generation mechanism in auditory sensory cells

Federico Kalinec; Matthew C. Holley; Kuni H. Iwasa; David J. Lim; Bechara Kachar

doi:10.1073/pnas.89.18.8671

A membrane-based force generation mechanism in auditory sensory cells

Federico Kalinec, Matthew C. Holley, Kuni H. Iwasa, David J. Lim, Bechara Kachar

Research output: Contribution to journal › Article › peer-review

218 Scopus citations

Abstract

Auditory outer hair cells can elongate and shorten at acoustic frequencies in response to changes of plasma membrane potential. We show that this fast bidirectional contractile activity consists of an electromechanical transduction process that occurs at the lateral plasma membrane and can be activated and analyzed independently in small membrane patches inside a patch electrode. Bidirectional forces are generated by increases and decreases in membrane area in response to hyperpolarization and depolarization, respectively. We suggest that the force generation mechanism is driven by voltage-dependent conformational changes within a dense array of large transmembrane proteins associated with the site of electromechanical transduction.

Original language	English (US)
Pages (from-to)	8671-8675
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	18
DOIs	https://doi.org/10.1073/pnas.89.18.8671
State	Published - 1992

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AU - Kalinec, Federico

AU - Holley, Matthew C.

AU - Iwasa, Kuni H.

AU - Lim, David J.

AU - Kachar, Bechara

PY - 1992

Y1 - 1992

N2 - Auditory outer hair cells can elongate and shorten at acoustic frequencies in response to changes of plasma membrane potential. We show that this fast bidirectional contractile activity consists of an electromechanical transduction process that occurs at the lateral plasma membrane and can be activated and analyzed independently in small membrane patches inside a patch electrode. Bidirectional forces are generated by increases and decreases in membrane area in response to hyperpolarization and depolarization, respectively. We suggest that the force generation mechanism is driven by voltage-dependent conformational changes within a dense array of large transmembrane proteins associated with the site of electromechanical transduction.

AB - Auditory outer hair cells can elongate and shorten at acoustic frequencies in response to changes of plasma membrane potential. We show that this fast bidirectional contractile activity consists of an electromechanical transduction process that occurs at the lateral plasma membrane and can be activated and analyzed independently in small membrane patches inside a patch electrode. Bidirectional forces are generated by increases and decreases in membrane area in response to hyperpolarization and depolarization, respectively. We suggest that the force generation mechanism is driven by voltage-dependent conformational changes within a dense array of large transmembrane proteins associated with the site of electromechanical transduction.

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A membrane-based force generation mechanism in auditory sensory cells

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