A lamb model for human respiratory syncytial virus infection.

Craig D. Lapin, Peter W. Hiatt, Claire Langston, Edward Mason, Pedro T. Piedra

    Research output: Contribution to journalArticlepeer-review

    21 Scopus citations

    Abstract

    Respiratory syncytial virus (RSV) is the most important cause of bronchiolitis and pneumonia in young children. The development of an animal model of RSV disease serves to better understanding the pathophysiology of airway disease from RSV infection in infants and children. Groups of six lambs were inoculated intratracheally (IT) or intranasally (IN) with a human strain of RSV (H-RSV). For controls 8 lambs received IT virus-free cell lysate. Tachypnea and fever were observed significantly more often following IT than following IN inoculation of H-RSV or IT placebo (for tachypnea: 20 of 69 days, 5 of 63 days, and 3 of 89 days, respectively, P < 0.001; for fever: 6 of 69 days, 0 of 63 days, and 1 of 89 days, respectively, P < 0.02). Nasal fluid production was significantly more frequent in both IT (14 of 69 days) and IN (15 of 63 days) groups than in the placebo group (2 of 87 days, P < 0.001). Postvaccination geometric mean titers (GMT, arithmetic transformation of log 2) of RSV-specific neutralizing antibody were significantly increased in the IT H-RSV group compared with postplacebo GMTs at 1 week (72 vs. 6.7, P < 0.03). By the second week postinoculation both H-RSV-infected groups had comparable levels of RSV-specific neutralizing antibody titers and had significantly greater GMTs for the second through to the fourth week than the placebo group (144, 128, and 4.8, respectively P < 0.0008). Bacterial isolates of the upper airway were comparable among the three groups. Histopathology at day 28 postinoculation was unremarkable for the three study groups.(ABSTRACT TRUNCATED AT 250 WORDS)

    Original languageEnglish (US)
    Pages (from-to)151-156
    Number of pages6
    JournalPediatric Pulmonology
    Volume15
    Issue number3
    DOIs
    StatePublished - Mar 1993

    Keywords

    • RSV‐specific antibody titer
    • Tachypnea
    • bacterial isolator
    • fever
    • nasal discharge

    ASJC Scopus subject areas

    • Pediatrics, Perinatology, and Child Health
    • Pulmonary and Respiratory Medicine

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