A direct measurment of the trapping time of lambda dna at an entropic barrier

J. D. Cross, K. T. Samiee, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Laser induced fluorescence and photon counting were used to measure the time lambda DNA molecules were delayed at entropic barriers in nanofluidic channels. In this work, we observe individual DNA molecules as they are delayed by, probe, and finally overcome entropic barriers. The sizes of DNA molecules and their flow speeds in fluidic channels can be quantified by measuring photon bursts collected when fluorescently labeled molecules pass through a focused laser [1,2]. Our ability to carefully measure the delay time for individual molecules represents an improvement over the indirect, or "bulk", methods previously used to determine the same quantity [3].

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages829-831
Number of pages3
Volume1
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • Entropic barrier
  • Entropic trap
  • Nanofluidic channel
  • Photon counting

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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