A direct measurment of the trapping time of lambda dna at an entropic barrier

J. D. Cross; K. T. Samiee; Harold G. Craighead

A direct measurment of the trapping time of lambda dna at an entropic barrier

J. D. Cross, K. T. Samiee, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Laser induced fluorescence and photon counting were used to measure the time lambda DNA molecules were delayed at entropic barriers in nanofluidic channels. In this work, we observe individual DNA molecules as they are delayed by, probe, and finally overcome entropic barriers. The sizes of DNA molecules and their flow speeds in fluidic channels can be quantified by measuring photon bursts collected when fluorescently labeled molecules pass through a focused laser [1,2]. Our ability to carefully measure the delay time for individual molecules represents an improvement over the indirect, or "bulk", methods previously used to determine the same quantity [3].

Original language	English (US)
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Transducer Research Foundation
Pages	829-831
Number of pages	3
Volume	1
ISBN (Print)	0974361119, 9780974361116
State	Published - Jan 1 2005
Event	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States Duration: Oct 9 2005 → Oct 13 2005

Other

Other	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country	United States
City	Boston, MA
Period	10/9/05 → 10/13/05

Keywords

Entropic barrier
Entropic trap
Nanofluidic channel
Photon counting

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

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A direct measurment of the trapping time of lambda dna at an entropic barrier. / Cross, J. D.; Samiee, K. T.; Craighead, Harold G.

Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. p. 829-831.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Cross, JD, Samiee, KT & Craighead, HG 2005, A direct measurment of the trapping time of lambda dna at an entropic barrier. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. vol. 1, Transducer Research Foundation, pp. 829-831, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 10/9/05.

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AB - Laser induced fluorescence and photon counting were used to measure the time lambda DNA molecules were delayed at entropic barriers in nanofluidic channels. In this work, we observe individual DNA molecules as they are delayed by, probe, and finally overcome entropic barriers. The sizes of DNA molecules and their flow speeds in fluidic channels can be quantified by measuring photon bursts collected when fluorescently labeled molecules pass through a focused laser [1,2]. Our ability to carefully measure the delay time for individual molecules represents an improvement over the indirect, or "bulk", methods previously used to determine the same quantity [3].

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