Abstract
Laser induced fluorescence and photon counting were used to measure the time lambda DNA molecules were delayed at entropic barriers in nanofluidic channels. In this work, we observe individual DNA molecules as they are delayed by, probe, and finally overcome entropic barriers. The sizes of DNA molecules and their flow speeds in fluidic channels can be quantified by measuring photon bursts collected when fluorescently labeled molecules pass through a focused laser [1,2]. Our ability to carefully measure the delay time for individual molecules represents an improvement over the indirect, or "bulk", methods previously used to determine the same quantity [3].
Original language | English (US) |
---|---|
Title of host publication | Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference |
Subtitle of host publication | 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
Publisher | Transducer Research Foundation |
Pages | 829-831 |
Number of pages | 3 |
Volume | 1 |
ISBN (Print) | 0974361119, 9780974361116 |
State | Published - Jan 1 2005 |
Event | 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States Duration: Oct 9 2005 → Oct 13 2005 |
Other
Other | 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 |
---|---|
Country/Territory | United States |
City | Boston, MA |
Period | 10/9/05 → 10/13/05 |
Keywords
- Entropic barrier
- Entropic trap
- Nanofluidic channel
- Photon counting
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering