TY - JOUR
T1 - A deep-sea hydrogen peroxide-stable alkaline serine protease from Aspergillus flavus
AU - Damare, Samir
AU - Mishra, Abhishek
AU - D’Souza-Ticlo-Diniz, Donna
AU - Krishnaswamy, Akhila
AU - Raghukumar, Chandralata
N1 - Publisher Copyright:
© 2020, King Abdulaziz City for Science and Technology.
PY - 2020/12
Y1 - 2020/12
N2 - We report here the production of an alkaline serine protease by Aspergillus flavus isolated at 5600-m depth from deep-sea sediments of the Central Indian Basin. When grown on defatted groundnut oil meal at 30 °C for 48–72 h, this fungal isolate produced 2000–2500 ACU mL−1 of alkaline protease. The purified protease had activity optima at pH 10.0 and 45 °C. It was a thiol-independent serine protease, identified as an alkaline serine protease ALP1 with a molecular mass of 42.57 kDa. The thermostability and activity of the enzyme increased at 60 °C, in the presence of additives such as sucrose, Tween 20, sorbitol, Ca2+ and glycerol and was not adversely affected by H2O2 indicating its potential as a detergent additive.
AB - We report here the production of an alkaline serine protease by Aspergillus flavus isolated at 5600-m depth from deep-sea sediments of the Central Indian Basin. When grown on defatted groundnut oil meal at 30 °C for 48–72 h, this fungal isolate produced 2000–2500 ACU mL−1 of alkaline protease. The purified protease had activity optima at pH 10.0 and 45 °C. It was a thiol-independent serine protease, identified as an alkaline serine protease ALP1 with a molecular mass of 42.57 kDa. The thermostability and activity of the enzyme increased at 60 °C, in the presence of additives such as sucrose, Tween 20, sorbitol, Ca2+ and glycerol and was not adversely affected by H2O2 indicating its potential as a detergent additive.
KW - Central Indian Basin
KW - Cold tolerant
KW - Deep-sea sediment
KW - Detergent
KW - Solid substrate
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U2 - 10.1007/s13205-020-02520-x
DO - 10.1007/s13205-020-02520-x
M3 - Article
AN - SCOPUS:85095820397
SN - 2190-572X
VL - 10
JO - 3 Biotech
JF - 3 Biotech
IS - 12
M1 - 528
ER -