A deep-sea hydrogen peroxide-stable alkaline serine protease from Aspergillus flavus

Samir Damare, Abhishek Mishra, Donna D’Souza-Ticlo-Diniz, Akhila Krishnaswamy, Chandralata Raghukumar

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We report here the production of an alkaline serine protease by Aspergillus flavus isolated at 5600-m depth from deep-sea sediments of the Central Indian Basin. When grown on defatted groundnut oil meal at 30 °C for 48–72 h, this fungal isolate produced 2000–2500 ACU mL−1 of alkaline protease. The purified protease had activity optima at pH 10.0 and 45 °C. It was a thiol-independent serine protease, identified as an alkaline serine protease ALP1 with a molecular mass of 42.57 kDa. The thermostability and activity of the enzyme increased at 60 °C, in the presence of additives such as sucrose, Tween 20, sorbitol, Ca2+ and glycerol and was not adversely affected by H2O2 indicating its potential as a detergent additive.

Original languageEnglish (US)
Article number528
Journal3 Biotech
Volume10
Issue number12
DOIs
StatePublished - Dec 2020

Keywords

  • Central Indian Basin
  • Cold tolerant
  • Deep-sea sediment
  • Detergent
  • Solid substrate

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science (miscellaneous)
  • Agricultural and Biological Sciences (miscellaneous)

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